Abstract
Monoclonal antibodies against a variety of antigens can be produced using techinques of somatic cell hybridization between cells of rodent myeloma lines and B cells derived from animals immunized against a given antigen1,2. However, because the monoclonal antibodies secreted by these hybridomas are of rodent origin, their use in human immunotherapy is limited. Thus the production of B-cell hybrids that secrete human monoclonal antibodies may be of considerable value. We have hybridized a hypoxanthine phosphoribosyl transferase (HPRT)-deficient human B-cell line derived from a patient suffering from multiple myeloma with peripheral lymphocytes obtained from a patient with subacute sclerosing panencephalitis (SSPE). These hybridomas were found to secrete human IgM specific for measles virus nucleocapsids.
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Croce, C., Linnenbach, A., Hall, W. et al. Production of human hybridomas secreting antibodies to measles virus. Nature 288, 488–489 (1980). https://doi.org/10.1038/288488a0
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DOI: https://doi.org/10.1038/288488a0
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