Abstract
Evidence has indicated that two soluble factors are essential for a T-cell proliferative response to antigen or lectin1–3. One such factor, thought to be of T-cell origin, is distinguished by its ability to initiate and maintain continuous T-cell growth (T-cell growth factor, TCGF)4–6. A second activity of macrophage origin augments lectin-initiated proliferative responses of thy-mocytes or adherent cell-depleted splenocytes (lymphocyte activating factor, LAF)7. Although it has been suggested that LAF promotes T-cell proliferation by facilitating the production of TCGF by T-cells1–3, direct evidence supporting this hypothesis has been lacking. We describe here experiments with a murine thymic lymphoma which releases TCGF in response to lectin. In serum-free, defined medium, highly purified LAF promotes a concentration-dependent increase in TCGF release. Because the proliferation of T cells depends on the concentration of TCGF available8,9, these results indicate that LAF and TCGF constitute an essential bimodal amplification system which ultimately determines the extent of T-cell clonal expansion.
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References
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Smith, K., Gilbride, K. & Favata, M. Lymphocyte activating factor promotes T-cell growth factor production by cloned marine lymphoma cells. Nature 287, 853–855 (1980). https://doi.org/10.1038/287853a0
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DOI: https://doi.org/10.1038/287853a0
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