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Human leukocyte interferon produced by E. coli is biologically active

Nature volume 287, pages 411–416 (1980)Cite this article

Abstract

A human leukocyte interferon cDNA was enzymatically synthesized, inserted into the vector pBR322, and cloned in Escherichia coli. The DNA sequence codes for a 23-amino acid signal peptide followed by an interferon polypeptide of 165 amino acids. An expression plasmid was constructed which permits the synthesis in E. coli of 2.5 x 108 units of interferon per litre of culture. This LeIF protected squirrel monkeys from lethal encephalomyocarditis virus infection.

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Authors and Affiliations

  1. Department of Molecular Biology, Genentech Inc., 460 Pt. San Bruno Blvd, South San Francisco, California, 94080

    David V. Goeddel, Elizabeth Yelverton, Axel Ullrich, Herbert L. Heyneker, Giuseppe Miozzari, William Holmes, Peter H. Seeburg & Thomas Dull

  2. Department of Biology, Genentech Inc., 460 Pt. San Bruno Blvd, South San Francisco, California, 94080

    Laurie May & Nowell Stebbing

  3. Department of Organic Chemistry, Genentech Inc., 460 Pt. San Bruno Blvd, South San Francisco, California, 94080

    Roberto Crea

  4. Roche Institue of Molecular Biology, Nutley, New Jersey, 07110

    Shuichiro Maeda, Russell McCandliss, Alan Sloma, John M. Tabor, Mitchell Gross, Phillip C. Familletti & Sidney Pestka

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  1. David V. Goeddel
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  8. Thomas Dull
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  9. Laurie May
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  12. Shuichiro Maeda
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  14. Alan Sloma
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  18. Sidney Pestka
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Goeddel, D., Yelverton, E., Ullrich, A. et al. Human leukocyte interferon produced by E. coli is biologically active. Nature 287, 411–416 (1980). https://doi.org/10.1038/287411a0

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