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Cooperative structural transition of PM2 DNA at high ionic strength and its dependence on DNA damages

Abstract

Pohl and Jovin1 have shown that poly (dG–dC) · poly (dG–dC) in solution undergoes a cooperative transition at 2.5 M NaCl at neutral pH. The two forms of the polymer are structurally different as indicated by a change in ethidium bromide intercalation and in circular dichroism (CD)1,2. Wang et al.3 and Davies and Zimmerman4 have suggested that the low salt form of the polymer might correspond to the B form of DNA (right-handed helix) and the high salt form to the Z form of DNA (left-handed helix). We describe here a salt-induced transition of supercoiled PM2 DNA from a form which passes through nitrocellulose filters to a form which is retained by these filters. The transition occurs between 2.5 and 3.5 M NaCl. The dependence of the apparent equilibrium constant on the salt concentration indicates a cooperative transition. Irradiation of DNA with UV light or alkylation with N-acetoxyacetylaminofluorene (AAAF) shifts the transition to lower salt concentrations. The transition can also be observed with linear DNA but requires a much higher salt concentration.

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