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Resolution, reconstitution and kinetics of the primary action of a hormone receptor

Naturevolume 287pages297300 (1980) | Download Citation

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Abstract

The β-adrenergic receptor (R) and the GTP binding protein (G) with which the receptor interacts were obtained in separate soluble fractions. Their recombination produced a hormone responsive system. In the presence of hormone the encounter of R with G in the reconstituted membrane occurs much faster than the subsequent R-induced activation of G.

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Affiliations

  1. Department of Biological Chemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

    • Yoav Citri
    •  & Michael Schramm

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https://doi.org/10.1038/287297a0

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