Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Resolution, reconstitution and kinetics of the primary action of a hormone receptor

Abstract

The β-adrenergic receptor (R) and the GTP binding protein (G) with which the receptor interacts were obtained in separate soluble fractions. Their recombination produced a hormone responsive system. In the presence of hormone the encounter of R with G in the reconstituted membrane occurs much faster than the subsequent R-induced activation of G.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Czech, M. P. A. Rev. Biochem. 46, 359–384 (1977).

    Article  CAS  Google Scholar 

  2. Heidmann, T. & Changeux, J.P. A. Rev. Biochem. 47, 317–357 (1978).

    Article  CAS  Google Scholar 

  3. Jones, L. M. & Michell, R. H. Biochem. Soc. Trans. 6, 673–688 (1978).

    Article  CAS  PubMed  Google Scholar 

  4. Rodbell, M. Nature 284, 17–22 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  5. Ross, E. M. & Gilman, A. G. A. Rev. Biochem. (in the press).

  6. Orly, J. & Schramm, M. Proc. natn. Acad. Sci. U.S.A. 73, 4410–4414 (1976).

    Article  ADS  CAS  Google Scholar 

  7. Vauquelin, G., Geynet, P., Hanoune, J. & Strosberg, D. Proc. natn. Acad. Sci. U.S.A. 74, 3710–3714 (1977).

    Article  ADS  CAS  Google Scholar 

  8. Rodbell, M., Birnbaumer, L., Pohl, S. L. & Krans, H. M. J. J. biol. Chem. 246, 1877–1882 (1971).

    CAS  PubMed  Google Scholar 

  9. Cassel, D. & Selinger, Z. Proc. natn. Acad. Sci. U.S.A. 75, 4155–4159 (1978).

    Article  ADS  CAS  Google Scholar 

  10. Pfeuffer, T. J. biol. Chem. 252, 7224–7234 (1977).

    CAS  PubMed  Google Scholar 

  11. Cassel, D., Levkovitz, H. & Selinger, Z. J. Cyclic Nucleotide Res. 3, 393–406 (1977).

    CAS  PubMed  Google Scholar 

  12. Schramm, M. & Rodbell, M. J. biol. Chem. 250, 2232–2237 (1975).

    CAS  PubMed  Google Scholar 

  13. Cautrecasas, P. & Hollenberg, M. D. Adv. Protein Chem. 30, 251–451 (1976).

    Article  Google Scholar 

  14. Maguire, M. E., Ross, E. M. & Gilman, A. G. Adv. Cyclic Nucleotide Res. 8, 1–83 (1977).

    CAS  PubMed  Google Scholar 

  15. Tolkovsky, A. M. & Levitzki, A. Biochemistry 17, 3795–3810 (1978).

    Article  CAS  PubMed  Google Scholar 

  16. Bourne, H. R., Coffino, P. & Tomkins, G. M. Science 187, 750–752 (1975).

    Article  ADS  CAS  PubMed  Google Scholar 

  17. Ross, E. M., Hewlett, A. C., Ferguson, K. M. & Gilman, A. G. J. biol. Chem. 253, 6401–6412 (1978).

    CAS  PubMed  Google Scholar 

  18. Neufeld, G., Schramm, M. & Weinberg, N. J. biol. Chem. (in the press).

  19. Eimerl, S., Neufeld, G., Korner, M. & Schramm, M. Proc. natn. Acad. Sci. U.S.A. 77, 760–764 (1980).

    Article  ADS  CAS  Google Scholar 

  20. Sternweis, P. C. & Gilman, A. G. J. biol. Chem. 254, 3333–3340 (1979).

    CAS  PubMed  Google Scholar 

  21. Cuatrecasas, P. A. Rev. Biochem. 43, 169–214 (1974).

    Article  CAS  Google Scholar 

  22. Schramm, M. J. Cyclic Nucleotide Res. 2, 347–358 (1976).

    CAS  PubMed  Google Scholar 

  23. Limbird, L. E., Gill, M. D. & Lefkowitz, R. J. Proc. natn. Acad. Sci. U.S.A. 77, 775–779 (1980).

    Article  ADS  CAS  Google Scholar 

  24. Minneman, K. P., Weiland, G. A. & Molinoflf, P. B. Molec. Pharmac. 17, 1–7 (1980).

    CAS  Google Scholar 

  25. Lad, P. M., Nielsen, T. B., Preson, S. M. & Rodbell, M. J. biol. Chem. 255, 988–995 (1980).

    CAS  PubMed  Google Scholar 

  26. Stadel, J. M., Delean, A. & Lefkowitz, R. J. J. biol. Chem. 255, 1436–1441 (1980).

    CAS  PubMed  Google Scholar 

  27. Weiland, G. A., Minneman, K. P. & Molinoff, P. B. Nature 281, 114–117 (1979).

    Article  ADS  CAS  PubMed  Google Scholar 

  28. Cassel, D. & Selinger, Z. Biochim. biophys. Acta 452, 538–551 (1976).

    Article  CAS  PubMed  Google Scholar 

  29. Orly, J. & Schramm, M. Proc. natn. Acad. Sci. U.S.A. 72, 3433–3437 (1975).

    Article  ADS  CAS  Google Scholar 

  30. Rimon, G., Hanski, E., Braun, S. & Levitzki, A. Nature 276, 394–396 (1978).

    Article  ADS  CAS  PubMed  Google Scholar 

  31. Rogers, M. J. & Strittmatter, P. J. biol. Chem. 249, 5565–5569 (1974); 250, 5713–5718 (1975).

    CAS  PubMed  Google Scholar 

  32. Schramm, M. Proc. natn. Acad. Sci. U.S.A. 76, 1174–1178 (1979).

    Article  ADS  CAS  Google Scholar 

  33. Salomon, Y., Londos, C. & Rodbell, M. Analyt. Biochem. 58, 541–548 (1974).

    Article  CAS  PubMed  Google Scholar 

  34. Maguire, M. E., Wiklund, R. A., Anderson, H. J. & Gilman, A. G. J. biol. Chem. 251, 1221–1231 (1976).

    CAS  PubMed  Google Scholar 

  35. Cassel, D. & Selinger, Z. J. Cyclic Nucleotide Res. 3, 11–22 (1977).

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Citri, Y., Schramm, M. Resolution, reconstitution and kinetics of the primary action of a hormone receptor. Nature 287, 297–300 (1980). https://doi.org/10.1038/287297a0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/287297a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing