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Expression of human fibroblast interferon gene in Escherichia coli

Naturevolume 287pages193197 (1980) | Download Citation

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Abstract

The human fibroblast Interferon gene was inserted in a thermoinducible expression plasmid under control of the phage lambda PL promotor. The primary translation products predicted on the basis of the plasmid constructions were hybrid proteins starting with β-lactamase or phage MS2 polymerase information followed by the total preinterferon. On induction, antiviral activity, whose physico-chemical, immunological and biological characteristics closely corresponded to those of authentic human fibroblast Interferon, was synthesized. Processing to a size compatible with mature but unglycosylated authentic product was observed.

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References

  1. 1

    Stewart, W. E. II The Interferon System (Springer, Berlin, 1979).

  2. 2

    Gresser, I. & Tovey, M. G. Biochim. biophys. Acta 516, 231–247 (1978).

  3. 3

    Bloom, B. R. Nature 284, 593–595 (1980).

  4. 4

    Desmyter, J. et al. Lancet ii, 645–647 (1976).

  5. 5

    Horoszewicz, J. S. et al. Cancer Treat. Rep. 62, 1899–1906 (1978).

  6. 6

    Sundmacher, R., Cantell, K., Skoda, K., Hallermann, C. & Neumann-Haefelin, D. Albrecht v. Graefes Arch. Klin. exp. Ophthal. 208, 229–233 (1978).

  7. 7

    Dolen, J. G. et al. Am. J. Med. 67, 127–131 (1979).

  8. 8

    Treuner, J. et al. Lancet i, 817–818 (1980).

  9. 9

    Gutterman, J. U. et al. Ann. int. Med. (in the press).

  10. 10

    Derynck, R. et al. Nature 285, 542–547 (1980).

  11. 11

    Nagata, S. et al. Nature 284, 316–320 (1980).

  12. 12

    Taniguchi, T. et al. Proc. Jap. Acad. 55, Ser B, 464–469 (1979).

  13. 13

    Sutcliffe, J. Cold Spring Harb. Symp. quant. Biol. 49, 77–90 (1978).

  14. 14

    Devos, R., Van Emmelo, J., Contreras, R. & Fiers, W. J. molec. Biol. 128, 595–619 (1979).

  15. 15

    Fiers, W. et al. Nature 260, 500–507 (1976).

  16. 16

    Greer, H. Virology 66, 589–604 (1975).

  17. 17

    Davey, M. W., Sulkowski, E. & Carter, W. A. J. biol. Chem. 251, 7620–7625 (1976).

  18. 18

    Knight, E., Jr Proc. natn. Acad. Sci. U.S.A. 73, 520–523 (1976).

  19. 19

    Stewart, W. E. II, De Clercq, E. & De Somer, P. Nature 249, 460–461 (1974).

  20. 20

    Kerr, I. M., Brown, R. E. & Hovanessian, A. G. Nature 268, 540–542 (1977).

  21. 21

    Neu, H. C. & Heppel, L. A. J. biol. Chem. 240, 3685–3692 (1965).

  22. 22

    Havell, E. A., Vilcek, J., Falcoff, E. & Berman, B. Virology 63, 475–483 (1975).

  23. 23

    Billiau, A., Joniau, M. & De Somer, P. J. gen. Virol. 19, 1–8, (1973).

  24. 24

    Kimchi, A. et al. Proc. natn. Acad. Sci. U.S.A. 76, 3208–3212 (1979).

  25. 25

    Minks, M. A., West, D. K., Benvin, S. & Baglioni, C. J. biol. Chem. 254, 10180–10183 (1979).

  26. 26

    Wang, R., Shedlarski, J., Farber, M., Kuebbing, D. & Ehrlich, M. Biochim. biophys. Acta (in the press).

  27. 27

    Sgaramella, V. & Ehrlich, S. Eur. J. Biochem. 86, 501–537 (1978).

  28. 28

    Laemmli, U. K. Nature 227, 680–682 (1970).

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Affiliations

  1. Laboratory of Molecular Biology, State University of Ghent, Ledeganckstraat 35, B-9000, Ghent, Belgium

    • Rik Derynck
    • , Erik Remaut
    • , Erik Saman
    • , Patrick Stanssens
    •  & Walter Fiers
  2. Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B-3000, Leuven, Belgium

    • Erik De Clercq
  3. Institut Pasteur du Brabant, rue du Remorqueur 28, B-1040, Brussels, Belgium

    • Jean Content

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https://doi.org/10.1038/287193a0

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