Letter | Published:

Gonadotropin releasing hormone agonists stimulate meiotic maturation of follicle-enclosed rat oocytes in vitro

Naturevolume 287pages145146 (1980) | Download Citation

Subjects

Abstract

Although the principal function of gonadotropin releasing hormone (GnRH) is to stimulate the pituitary gland to release luteinizing hormone (LH) and follicle-stimulating hormone (FSH), there is evidence that agonistic analogues of GnRH directly inhibit steroidogenesis in the testis1 and ovary2–5. On the other hand, Clark et al.6 have demonstrated that GnRH and two agonists have a marked stimulatory effect on prostaglandin synthesis by granulosa cells isolated from immature rats treated with pregnant mare's serum gonadotropin (PMSG). Stimulation by these compounds was distinct from that by LH in that no changes in cyclic AMP production were detected. Thus it seems important to investigate the effect of these peptides on other aspects of ovarian function, for example oocyte maturation. Mammalian oocytes are arrested in the dictyate stage of the first meiotic prophase, and meiosis (oocyte maturation) normally resumes in preovulatory follicles as a consequence of the surge of LH and FSH. This maturation can also be initiated in vitro by the addition of gonadotropins to isolated preovulatory follicles7,8, and is accompanied by an increase in the production of lactate8. We now report that GnRH and two potent agonists9 stimulate meiosis in vitro in follicle-enclosed oocytes in a dose-dependent and specific manner, and also cause an increase of lactate accumulation during incubation.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Hsueh, A. J. W. & Erickson, G. F. Nature 281, 66–67 (1979).

  2. 2

    Rippel, R. H. & Johnson, E. S. Proc. Soc. exp. Biol. Med. 152, 432–437 (1976).

  3. 3

    Hsueh, A. J. W. & Erickson, G. F. Science 204, 854–855 (1979).

  4. 4

    Ying, S-Y. & Guillemin, R. Nature 280, 593–595 (1979).

  5. 5

    Clayton, R. N., Harwood, S. P. & Catt, K. J. Nature 282, 90–92 (1979).

  6. 6

    Clark, M. R., Thibier, C., Marsh, J. M. & LeMaire, W. J. Endocrinology (in the press).

  7. 7

    Tsafriri, A., Linder, H. R., Zor, U. & Lamprecht, S. A. J. Reprod. Fert. 31, 39–50 (1972).

  8. 8

    Hillensjö, T. Acta endocr. (Kbh). 82, 809–830 (1976).

  9. 9

    Vale, W., Rivier, C. & Brown, M. A. A. Rev. Physiol. 39, 473–527 (1977).

  10. 10

    Nilsson, L. Acta endocr. (Kbh) 77, 540–558 (1974).

  11. 11

    Donahue, R. P. J. exp. Zool. 169, 237–250 (1968).

  12. 12

    Hillensjö, T., Dekel, N. & Ahrén, K. Acta physiol. scand. 96, 558–568 (1976).

  13. 13

    Erickson, G. F. & Hsueh, A. J. W. Soc. gynec. Invest. 27th A. Meet., Denver, Abstr. 371 (1980).

  14. 14

    Lindner, H. R. et al. Recent Prog. Horm. Res. 30, 79–127 (1974).

  15. 15

    Tsafriri, A., Pomerantz, S. H. & Channing, C. P. Biol. Reprod. 14, 511–516 (1976).

  16. 16

    Aksel, S. & Glass, R. Am. J. Obstet. Gynec. 134, 619–623 (1979).

  17. 17

    Siler-Khodr, T. M. & Khodr, G. S. Am. J. Obstet. Gynec. 130, 216–219 (1978).

  18. 18

    Ying, S-Y & Guillemin, R. C. r. hebd. Séanc. Acad. Sci. Paris 289D, 943–946 (1979).

  19. 19

    Harwood, J. P., Clayton, R. N. & Catt, K. J. 6th Int. Cong. Endocr., Melbourne, Abstr. 813 (1980).

  20. 20

    Rodbard, D., Moyle, E. & Ramanchandran, J. Curr. Topics molec. Endocr. 1, 79–87 (1974).

Download references

Author information

Affiliations

  1. Department of Physiology, University of Göteborg, S-40033, Göteborg, Sweden

    • T. Hillensjö
    •  & W. J. LeMaire
  2. Department of Obstetrics and Gynecology and the Endocrine Laboratory, University of Miami, School of Medicine, Miami, Florida

    • W. J. LeMaire

Authors

  1. Search for T. Hillensjö in:

  2. Search for W. J. LeMaire in:

About this article

Publication history

Received

Accepted

Issue Date

DOI

https://doi.org/10.1038/287145a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.