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Structure of a family of rat amylase genes

Naturevolume 287pages117122 (1980) | Download Citation

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Abstract

The sequences of two cloned rat pancreatic amylase cDNAs comprising 95% of the mRNA sequence are reported. Analysis of cloned rat genomic DNA fragments using cloned cDNA probes indicates that the rat genome contains multiple closely related amylase genes in which the cDNA sequences are distributed within a region 9 kilobases in length and are interrupted by at least seven intervening sequences.

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References

  1. 1

    Sanders, T. G. thesis, Univ. Illinois (1970).

  2. 2

    Loyter, A. & Schramm, M. Biochim. biophys. Acta 65, 200 (1962).

  3. 3

    Arnold, M. & Rutter, W. J. J. biol. Chem. 238, 2760–2765 (1963).

  4. 4

    Rutter, W. J., Wessells, N. K. & Grobstein, C. Natn. Cancer Inst. Monogr. 13, 51–65 (1964).

  5. 5

    Kemp, J. D., Walther, B. T. & Rutter, W. J. J. biol. Chem. 247, 3941–3952 (1972).

  6. 6

    Sanders, T. G. & Rutter, W. J. J. biol. Chem. 249, 3500–3509 (1974).

  7. 7

    Van Nest, G. A., MacDonald, R. J., Raman, R. K. & Rutter, W. J. J. Cell Biol. (in the press).

  8. 8

    Harding, J. D. et al. J. biol. Chem. 252, 7391–7397 (1977).

  9. 9

    Przbyla, A. E., MacDonald, R. J., Harding, J. D., Pictet, R. L. & Rutter, W. J. J. biol. Chem. 254, 2154–2159 (1979).

  10. 10

    Rutter, W. J., Ball, W. D., Bradshaw, W. S., Clark, W. R. & Sanders, T. G. in Secretory Mechanisms of Salivary Glands (eds Schneyer, L. H. & Schneyer, C. A.) 238–253 (Academic, New York, 1967).

  11. 11

    Palla, J. C., Abdeljlil, A. B. & Desnuelle, P. Biochim. biophys. Acta 158, 25–35 (1968).

  12. 12

    Rall, L. B., Pictet, R., Githens, S. & Rutter, W. J. J. Cell Biol. 75, 398–409 (1977).

  13. 13

    Harding, J. D. & Rutter, W. J. J. biol. Chem. 253, 8736–8740 (1978).

  14. 14

    Malacinski, G. M. & Rutter, W. J. Biochemistry 8, 4382–4390 (1969).

  15. 15

    Sanders, T. G. & Rutter, W. J. Biochemistry 11, 130–136 (1972).

  16. 16

    McGeachin, R. L. & Reynolds, J. M. J. biol. Chem. 234, 1456–1400 (1959); Biochim. biophys. Acta 39, 531–500 (1960).

  17. 17

    MacDonald, R. J. et al. (in preparation).

  18. 18

    Maxam, A. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).

  19. 19

    Bailey, J. M. & Davidson, N. Analyt. Biochem. 70, 75–85 (1976).

  20. 20

    Lomedico, P. et al. Cell 18, 545–558 (1979).

  21. 21

    Vandermeers, A. & Christophe, J. Biochim. biophys. Acta 154, 110–129 (1968).

  22. 22

    Dayhoff, M. O., Hunt, L. T. & Hurst-Calderone, S. in Atlas of Protein Sequence and Structure Vol. 5, Suppl. 3 (ed. Dayhoff, M. O.) 363 (National Biomedical Research Foundation, Washington DC, 1978).

  23. 23

    Ambler, R. P. Meth. Enzym. 25, 262–272 (1972).

  24. 24

    Petra, D. H. Meth. Enzym. 19, 460–503 (1970).

  25. 25

    Cozzone, P., Pasero, L., Beaupoil, B. & Marchis-Mouren, G. Biochim. biophys. Acta 207, 490–504 (1970).

  26. 26

    Gorecki, M. & Zeelon, E. P. J. biol. Chem. 254, 525–529 (1979).

  27. 27

    Blobel, G. & Dobberstein, B. J. Cell Biol. 67, 835–851 (1975).

  28. 28

    Baralle, F. E. & Borwnlee, G. G. Nature 274, 84–87 (1978).

  29. 29

    Nakanishi, S. et al. Nature 278, 423–427 (1979).

  30. 30

    Ullrich, A. et al. Science 196, 1313–1319 (1977).

  31. 31

    Nunberg, J. H., Kaufman, R. J., Chang, A. C. Y., Cohen, S. N. & Schimke, R. T. Cell 19, 355–364 (1980).

  32. 32

    Hobart, P., Crawford, R., Shen, L.-P., Pictet, R. & Rutter, W. J. Nature (in the press).

  33. 33

    Levitzki, A., Heller, J. & Schramm, M. Biochim. biophys. Acta 81, 101–107 (1964).

  34. 34

    Loyter, A. & Schramm, M. J. biol. Chem. 241, 2611–2617 (1966).

  35. 35

    Mora, S., Simon, I. & Elodi, P. Molec. Cell Biochem. 4, 205–209 (1974).

  36. 36

    Robyt, J. F., Chittenden, C. G. & Lee, C. T. Archs. Biochem. Biophys. 144, 160–167 (1971).

  37. 37

    Lehrner, L. N. & Malacinski, G. M. in Isozymes Vol. 4 (ed. Market, C. L.) 727–743 (Academic, New York, 1975).

  38. 38

    Simon, I., Mora, S. & Elodi, P. Molec. Cell Biochem. 4, 211–216 (1974).

  39. 39

    Fitzgerald, P. M. D., Stankiewicz, P. J., Smith, S. C. & McPherson, A. J. molec. Biol. 135, 753–756 (1979).

  40. 40

    Payan, F., Haser, R., Pierrot, M., Frey, M. & Astier, J. P. Acta crystallogr. B36, 416–421 (1980).

  41. 41

    McLachlan, A. D. J. molec. Biol. 61, 409–424 (1971).

  42. 42

    Garnier, J., Osguthorpe, D. J. & Robson, B. J. molec. Biol. 120, 97–120 (1978).

  43. 43

    Chou, P. Y. & Fasman, G. D. Biochemistry 13, 222–245 (1974).

  44. 44

    Tang, J., James, M. N. G., Hsu, I. N., Jenkins, J. A. & Blundell, T. L. Nature 271, 618–721 (1978).

  45. 45

    Ploegman, J. H., Drent, G., Kalk, K. H. & Hel, W. G. J. J. molec. Biol. 123, 557–594 (1978).

  46. 46

    McLachlan, A. D. J. molec. Biol. 128, 49–79 (1979).

  47. 47

    Sargent, T. D. et al. Proc. natn. Acad. Sci. U.S.A. 76, 3256–3260 (1979).

  48. 48

    Benton, W. D. & Davis, R. W. Science 196, 180–182 (1977).

  49. 49

    Breathnach, R., Benoist, C., O'Hare, K., Gannon, F. & Chambon, P. Proc. natn. Acad. Sci. U.S.A. 75, 4853–4857 (1978).

  50. 50

    Dugaiczyk, A. et al. Nature 274, 328–333 (1978).

  51. 51

    Cordell, B. et al. Cell 18, 533–543 (1979).

  52. 52

    Bell, G. I. et al. Nature 284, 26–32 (1980).

  53. 53

    Tsujimoto, Y. & Suzuki, Y. Cell 18, 591–600 (1979).

  54. 54

    Goodman, H. M. & MacDonald, R. J. Meth. Enzym. 68, 75–79 (1979).

  55. 55

    Fergusson, J. & Davis, R. W. in Advanced Techniques in Biological Electron Microscopy (ed. Koehler, J. K.) 123–171 (Springer, Berlin,1978).

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Author notes

    • Raymond J. MacDonald

    Present address: Department of Biochemistry, University of Texas Health Science Center, 5323 Harry Hines Blvd, Dallas, Texas, 75235

    • Michael M. Crerar

    Present address: BioLogicals, Inc.,Department of Biology, York University, Downs-view, Ontario, Canada, M35 1P3

    • Raymond L. Pictet
    •  & Gilles Thomas

    Present address: Institut de Biologie Moleculaire, Université Paris VII, Tour 43, 75221, Paris, Cedex 05, France

Affiliations

  1. Department of Biochemistry and Biophysics, University of California, San Francisco, California, 94143

    • Raymond J. MacDonald
    • , Michael M. Crerar
    • , William F. Swain
    • , Raymond L. Pictet
    • , Gilles Thomas
    •  & William J. Rutter

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https://doi.org/10.1038/287117a0

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