Letter | Published:

Involvement of the B-lymphoid system in chronic myelogenous leukaemia

Subjects

Abstract

Studies with glucose-6-phosphate dehydrogenase (G6PD) iso-enzymes have demonstrated that chronic myelogenous leukaemia (CML) is a clonal disorder of pluripotent haematopoietic stem cells which are capable of differentiation to myeloid cells, monocytes, erthrocytes and platelets1. It has been observed recently in G6PD heterozygous patients with chronic phase CML that the non-E-rosetting lymphocytes were restricted to a single enzyme type, indicating that some lymphoid cells must also arise from the leukaemic clone2. Surface or cytoplas-mic immunoglobulin could be detected in up to 46% of the cells of these isolated non-T-lymphocyte populations, which suggested that cells from the CML clone were capable of differentiating into B lymphocytes. To investigate this further, we established Epstein–Barr virus (EBV)-transformed B-lymphoblastoid cell lines derived from patients with CML and studied chromosomes and G6PD to determine whether progenitor B lymphocytes for any of the cell lines had originated from the CML clone. We report here direct evidence that immunoglobulin-synthesizing B lymphocytes can arise from the CML stem cell clone.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Fialkow, P. J., Jacobson, R. J. & Papayannopoulou, T. Am J. Med. 63, 125–130 (1977).

  2. 2

    Fialkow, P. J., Denman, A. M., Jacobson, R. J. & Lowenthal, M. N. J. clin. Invest. 62, 815–823 (1978).

  3. 3

    Miller, G. & Lipman, M. Proc. natn. Acad. Sci. U.S.A. 70, 190–194 (1973).

  4. 4

    Lozzio, C. B. & Lozzio, B. B. Blood 45, 321–334 (1975).

  5. 5

    Minowada, J., Koshiba, H., Janossy, G., Greaves, M. F. & Bollum, F. J. Leukaemia Res. 3, 261–266 (1979).

  6. 6

    Anderson, L. D., Jokinen, M. & Gahmberg, C. G. Nature 278, 364–365 (1979).

  7. 7

    Hurley, J. N., Fu, S. M., Kunkel, H. G., Chaganti, R. S. K. & German, J. Nature 283, 76–78 (1980).

  8. 8

    Lebien, T. W., Hozier, J., Minowada, J. & Kersey, J. H. New Engl. J. Med. 301, 144–147 (1979).

  9. 9

    Vogler, L. B., Crist, W. M., Vinson, A. S., Brattain, M. G. & Coleman, M. S. Blood 54, 1164–1170 (1979).

  10. 10

    Greaves, M. F. et al. Leukaemia Res. 3, 181–191 (1979).

  11. 11

    Sumner, A. T., Evans, H. J. & Buckland, R. A. Nature new Biol. 232, 31–32 (1971).

  12. 12

    Paris Conference: Standardization in Human Cytogenetics (The National Foundation—March of Dimes BD, OAS VIII, 1972).

  13. 13

    Paris Conference (1971) & Suppl. (1975>) (The National Foundation-March of Dimes BD, OAS XI No. 9, 1975).

  14. 14

    Hijmans, W., Schuit, H. J. R. & Klein, F. clin. exp. Immun. 4, 457–472 (1969).

  15. 15

    Fialkow, P. J. Ann. hum. Genet. 37, 39–48 (1973).

Download references

Author information

Rights and permissions

To obtain permission to re-use content from this article visit RightsLink.

About this article

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.