Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Deletion of immunoglobulin heavy chain genes from expressed allelic chromosome

Nature volume 286, pages 850–853 (1980)Cite this article

Abstract

We have studied the organization of immunoglobulin heavy-chain genes in a γ2b-chain (BALB/c allotype)-producing myeloma BKC F1 #15 induced in a F1 mouse between C57BL and BALB/c. Southern blot hybridization studies using cloned μ, γ1 and γ2b-chain genes as probes demonstrate that the μ- and γ1-chain genes of the expressed chromosome are deleted while these genes of the unexpressed chromosome are retained. The γ2b-chain gene of the expressed allele is rearranged while that gene of the unexpressed allele seems unchanged, as do the γ2a-chain genes. These results support the allelic deletion mechanism in heavy-chain class switch and the order of H chain genes.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Dreyer, W. J. & Bennett, J. C. Proc. natn. Acad. Sci. U.S.A. 54, 864–869 (1965).

    Article  ADS  CAS  Google Scholar 

  2. Tonegawa, S., Brack, C., Hozumi, N. & Schuller, R. Proc. natn. Acad. Sci. U.S.A. 74, 3518–3522 (1977).

    Article  ADS  CAS  Google Scholar 

  3. Brack, C., Hirama, M., Lenhard-Schuller, R. & Tonegawa, S. Cell 15, 1–14 (1978).

    Article  CAS  Google Scholar 

  4. Honjo, T. in Immunology '78 (eds Yamamura, Y. & Tada, T.) 75–82 (Nakayama-shoten, Tokyo, 1978).

    Google Scholar 

  5. Seidman, J. G. & Leder, P. Nature 276, 790–795 (1978).

    Article  ADS  CAS  Google Scholar 

  6. Seidman, J. G. et al. Proc. natn. Acad. Sci. U.S.A. 75, 3881–3885 (1978).

    Article  ADS  CAS  Google Scholar 

  7. Early, P. W., Davis, M. M., Kaback, D. B., Davidson, N. & Hood, L. Proc. natn. Acad. Sci. U.S.A. 76, 857–861 (1979).

    Article  ADS  CAS  Google Scholar 

  8. Honjo, T. et al. Cell 18, 559–568 (1979).

    Article  CAS  Google Scholar 

  9. Kataoka, T., Yamawaki-Kataoka, Y., Yamagishi, H. & Honjo, T. Proc. natn. Acad. Sci. U.S.A. 76, 4240–4244 (1979).

    Article  ADS  CAS  Google Scholar 

  10. Kemp, D. J., Cory, S. & Adams, J. M. Proc. natn. Acad. Sci. U.S.A. 76, 4627–4631 (1979).

    Article  ADS  CAS  Google Scholar 

  11. Sakano, H. et al. Nature 277, 627–633 (1979).

    Article  ADS  CAS  Google Scholar 

  12. Sakano, H., Hüppi, K., Heinrich, G. & Tonegawa, S. Nature 280, 288–294 (1979).

    Article  ADS  CAS  Google Scholar 

  13. Max, E. E., Seidman, J. G. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 76, 3450–3454 (1979).

    Article  ADS  CAS  Google Scholar 

  14. Rabbitts, T. H. Nature 275, 291–296 (1978).

    Article  ADS  CAS  Google Scholar 

  15. Dreyer, W. J., Gray, W. R. & Hood, L. Cold Spring Harb. Symp. quant. Biol. 32, 353–367 (1968).

    Article  Google Scholar 

  16. Gally, J. A. & Edelman, G. M. Nature 227, 341–342 (1970).

    Article  ADS  CAS  Google Scholar 

  17. Kabat, D. Science 175, 134–140 (1972).

    Article  ADS  CAS  Google Scholar 

  18. Sledge, C., Fair, D. S., Black, B., Kruegar, R. G. & Hood, L. Proc. natn. Acad. Sci. U.S.A. 73, 923–927 (1976).

    Article  ADS  CAS  Google Scholar 

  19. Tonegawa, S., Hozumi, N., Matthyssens, G. & Schuller, R. Cold Spring Harb. Symp. quant. Biol. 41, 877–889 (1976).

    Article  Google Scholar 

  20. Honjo, T. & Kataoka, T. Proc. natn. Acad. Sci. U.S.A. 75, 2140–2144 (1978).

    Article  ADS  CAS  Google Scholar 

  21. Rabbitts, T. H., Forster, A., Dunnick, W. & Bentley, D. L. Nature 283, 351–356 (1980).

    Article  ADS  CAS  Google Scholar 

  22. Kataoka, T., Kawakami, T., Takahashi, N. & Honjo, T. Proc. natn. Acad. Sci. U.S.A. 177, 919–923 (1980).

    Article  ADS  Google Scholar 

  23. Yamawaki-Kataoka, Y., Kataoka, T., Takahashi, N., Obata, M. & Honjo, T. Nature 283, 786–789 (1980).

    Article  ADS  CAS  Google Scholar 

  24. Kawakami, T., Takahashi, N. & Honjo, T. Nucleic Acids Res. (submitted).

  25. Yamawaki-Kataoka, Y. et al. Biochemistry 18, 490–494 (1979).

    Article  CAS  Google Scholar 

  26. Lieberman, R. Springer Seminars in Immunopath. 1, 7–30 (1978).

    Article  Google Scholar 

  27. Sibinovic, K. H., Potter, M., D'Hoostelaere, L., Robe, B. & Wax, J. Catalogue of Plasmacytomas and Other Tumors of the Lymphoreticular System, third edn (Litton Bionetics, Maryland 1976).

    Google Scholar 

  28. Yaoita, Y. & Honjo, T. Biomed. Res. 1, 164–175 (1980).

    Article  CAS  Google Scholar 

  29. Lenhard-Schuller, R., Hohn, B., Brack, C., Hirama, M. & Tonegawa, S. Proc. natn. Acad. Sci. U.S.A. 75, 4709–4713 (1978).

    Article  ADS  CAS  Google Scholar 

  30. Steinmetz, M., Zachau, H. G. & Mach, B. Nucleic Acid Res. 6, 3213–3229 (1979).

    Article  CAS  Google Scholar 

  31. Yoshida, T. H., Imai, H. T. & Potter, M. J. natn. Cancer Inst. 41, 1083–1095 (1968).

    Google Scholar 

  32. Southern, E. M. J. molec. Biol. 98, 503–517 (1975).

    Article  CAS  Google Scholar 

  33. Maniatis, T., Jeffreys, A. & Kleid, D. G. Proc. natn. Acad. Sci. U.S.A. 72, 1184–1188 (1975).

    Article  ADS  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Yoshio Yaoita & Tasuku Honjo

    Present address: Department of Genetics, Osaka University School of Medicine, Osaka, 530, Japan

Authors and Affiliations

  1. Department of Physiological Chemistry and Nutrition, Faculty of Medicine, University of Tokyo, Tokyo, 113, Japan

    Yoshio Yaoita & Tasuku Honjo

Authors
  1. Yoshio Yaoita
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tasuku Honjo
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yaoita, Y., Honjo, T. Deletion of immunoglobulin heavy chain genes from expressed allelic chromosome. Nature 286, 850–853 (1980). https://doi.org/10.1038/286850a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/286850a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing