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Free calcium in heart muscle at rest and during contraction measured with Ca2+-sensitive microelectrodes

Nature volume 286, pages 845850 (28 August 1980) | Download Citation

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Abstract

Direct measurements of free Ca2+ in heart cells are needed for an understanding of the regulation of contractility. We developed and used Ca2+-sensitive microelectrodes with fine tips, stable properties and ample sensitivity to free Ca2+ in the sub-micromolar range. In quiescent ventricular muscle, measurements which passed tests for electrode sealing and cell viability gave a mean free Ca2+ concentration of 0.26 µM. During contractures, we recorded Ca2+ transients rising as high as 10 µM. In studying the effects of catecholamines on free Ca2+ and force, we found evidence that adrenaline can reduce myofibrillar Ca2+ sensitivity in intact heart muscle.

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Author information

Author notes

    • Timothy J. Rink
    •  & Roger Y. Tsien

    Physiological Laboratory, University of Cambridge, Cambridge CB2 3EG, UK

Affiliations

  1. Department of Physiology, Yale University School of Medicine, New Haven, Connecticut 06510

    • Eduardo Marban
    • , Timothy J. Rink
    • , Richard W. Tsien
    •  & Roger Y. Tsien

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https://doi.org/10.1038/286845a0

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