Letter | Published:

Crystal structure of a ribosomal component at 2.6 Å resolution

Nature volume 286, pages 824826 (21 August 1980) | Download Citation

Subjects

Abstract

Structural information about the ribosomes has been sought in many ways1,2. It is known that the 50S subunit of Escherichia coli ribosomes contains four copies of the ribosomal protein L7/L12 (refs 3–7) in a unique region8,9. A complex can be isolated consisting of those four copies bound to another ribosomal protein, L10 (refs 10–13). L7/L12 aggregates into elongated dimers in solution14,15. Ribosomes deprived of L7/L12 have a reduced affinity for supernatant factors such as EF-Tu and EF-G and almost no capacity for factor-dependent GTP hydrolysis16–18. The sequence of the 120 amino acid residues in L7/L12 from E. coli has been determined19, and two domains of L7/L12 (residues 1–36 and 53–120, respectively) have been crystallized separately20. We now report the first crystal structure of a ribosomal component, a C-terminal fragment (CTF) of the protein L7/L12 from E. coli, determined to 2.6 Å resolution. The CTF has a compact, plum-shaped tertiary structure with a high content of secondary structure. The three α-helices and three β-strands in CTF account for 32% α-helix and 14% β-structure in the intact L7/L12 protein. A binding site for anions shows some resemblance to the known nucleotide binding site in many proteins.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1.

    A. Rev. Biochem. 46, 173–200 (1977).

  2. 2.

    Int. Rev. Biochem. 25, 121–177 (1979).

  3. 3.

    , & FEBS Lett. 8, 192–196 (1970).

  4. 4.

    in Ribosomes (eds Nomura, M., Tissières, A. & Lengyel, P.) 711–731 (Cold Spring Harbor, New York, 1974).

  5. 5.

    , , & in Ribosomes (eds Chambliss, G. et al.) 297–332 (University Park, Baltimore, 1979).

  6. 6.

    Molec. gen. Genet. 140, 253–274 (1975).

  7. 7.

    J. molec. Biol. 95, 1–8 (1975).

  8. 8.

    , & J. molec. Biol. 126, 123–140 (1978).

  9. 9.

    , & J. molec. Biol. 107, 479–490 (1976).

  10. 10.

    , & FEBS Lett. 64, 135–138 (1976).

  11. 11.

    & FEBS Lett. 98, 139–144 (1979).

  12. 12.

    Nucleic Acids Res. 6, 2637–2646 (1979).

  13. 13.

    , , & FEBS Lett. 109, 34–38 (1980).

  14. 14.

    & Biochem. biophys. Res. Commun. 61, 178–184 (1974).

  15. 15.

    , , & FEBS Lett. 66, 48–51 (1976).

  16. 16.

    , & J. biol. Chem. 247, 805–814 (1972).

  17. 17.

    , & Biochem. biophys. Res. Commun. 47, 866–873 (1972).

  18. 18.

    , , & FEBS Lett. 73, 6–11 (1977).

  19. 19.

    , , & Eur. J. Biochem. 34, 138–152 (1973).

  20. 20.

    , , & FEBS Lett. 88, 300–304 (1978).

  21. 21.

    , & J. molec. Biol. 90, 143–152 (1974).

  22. 22.

    , & Acta crystallogr. A24, 351–359 (1968).

  23. 23.

    Acta crystallogr. 20, 82–86 (1966).

  24. 24.

    & Acta crystallogr. 12, 794–802 (1959).

  25. 25.

    J. appl. Crystallogr. 11, 268–272 (1978).

  26. 26.

    J. molec. Biol. 75, 295–302 (1973).

  27. 27.

    , & 7th Aharon Katzir-Katchalsky Conf. (in the press).

  28. 28.

    FEBS Lett. 8, 197–200 (1970).

  29. 29.

    , , & Archs Biochem. Biophys. 198, 53–59 (1979).

  30. 30.

    , & Eur. J. Biochem. 90, 313–318 (1978).

  31. 31.

    & Biochemistry 19, 178–183 (1980).

  32. 32.

    , , & FEBS Lett. 34, 55–57 (1973).

  33. 33.

    , & FEBS Lett. 81, 204–213 (1977).

  34. 34.

    , , & FEBS Lett. 82, 125–129 (1977).

  35. 35.

    & Biochemistry 18, 2019–2027 (1979).

  36. 36.

    & Molec. cell. Biochem. 21, 161–182 (1978).

  37. 37.

    & Proc. natn. Acad. Sci. U.S.A. (in the press).

  38. 38.

    , , & Biochem. biophys. Res. Commun. 64, 1184–1191 (1975).

  39. 39.

    , & Eur. J. Biochem. 90, 319–323 (1978).

  40. 40.

    in Atlas of Protein Sequence and Structure (National Biomedical Research Foundation, Silver Spring, Maryland, 1972).

Download references

Author information

Affiliations

  1. Institute of Molecular Biology, Uppsala University, Box 562, S-751 22 Uppsala, Sweden

    • Marie Leijonmarck
    • , Stig Eriksson
    •  & Anders Liljas

Authors

  1. Search for Marie Leijonmarck in:

  2. Search for Stig Eriksson in:

  3. Search for Anders Liljas in:

About this article

Publication history

Received

Accepted

Published

DOI

https://doi.org/10.1038/286824a0

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.