Abstract
A mutant immunoglobulin gene has been formed by an abnormal (non V/J) recombination event such that abnormal RNA splicing is required to form a mutant light chain. The structure of the gene suggests that the small palindrome thought to be involved in V/J joining also provides the basis for this abnormal DNA recombination and that the absence of a J segment and RNA splice signal allows an abnormal RNA splicing reaction to occur.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Dreyer, W. J. & Bennett, J. C. Proc. natn. Acad. Sci. U.S.A. 54, 861–868 (1965).
Hozumi, N. & Tonegawa, S. Proc. natn. Acad. Sci. U.S.A. 73, 3628–3632 (1976).
Rabbitts, T.H. & Forster, A. Cell 13, 319–327 (1978).
Seidman, J. G. et al. Proc. natn. Acad. Sci. U.S.A. 75, 3881–3885 (1978).
Brack, G., Hirama, M., Lenhard-Schuller, R. & Tonegawa, S. Cell 15, 1–14 (1978).
Seidman, J. G. & Leder, P. Nature 276, 790–795 (1978).
Seidman, J. G., Max, E. E. & Leder, P. Nature 280, 370–375 (1979).
Max, E. E., Seidman, J.G. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 76, 3450–3454 (1979).
Sakano, H., Hüppi, K., Heinrich, G. & Tonegawa, S. Nature 280, 288–294 (1979).
Kuehl, W. M. & Scharff, M. D. J. molec. Biol. 89, 409–421 (1974).
Kuehl, W. M. et al. Cell 5, 139–147 (1975).
Rose, S. M., Kuehl, W. M. & Smith, G. P. Cell 12, 453–462 (1977).
Burstein, Y. & Schechter, I. Proc. natn. Acad. Sci. U.S.A. 74, 716–720 (1977).
Burstein, Y. & Schechter, I. Biochem. J. 157, 145–151 (1976).
Choi, E., Kuehl, M. & Wall, R. Nature 286, 776–779 (1980).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
Polsky, F., Edgell, M., Seidman, J. & Leder, P. Analyt. Biochem. 87, 397–410 (1978).
Tiemeier, D., Tilghman, S. & Leder, P. Gene 2, 173–191 (1977).
Maxam, A. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).
McKean, D., Potter, M. & Hood, L. Biochemistry 12, 794–759 (1973).
Weigert, M., Gatmaitan, L., Loh, E., Schilling, J. & Hood, J. Nature 276, 785–790 (1978).
Seidman, J. G., Leder, A., Nau, M., Norman, B. & Leder, P. Science 202, 11–17 (1978).
Bernard, O., Hozumi, N. & Tonegawa, S. Cell 15, 1133–1144 (1978).
Perry, R. P. et al. Proc. natn. Acad. Sci. U.S.A. (in the press).
Seidman, J. et al. Proc. natn. Acad. Sci. U.S.A. (in the press).
Berk, A. & Sharp, P.A. Cell 14, 695–711 (1978).
Kitchingman, G.R., Lai, S.P. & Westphal, H. Proc. natn. Acad. Sci. U.S.A. 74, 4392–4395 (1977).
Gelinas, R. E. & Roberts, R.J. Cell 11, 533–544 (1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Seidman, J., Leder, P. A mutant immunoglobulin light chain is formed by aberrant DNA- and RNA-splicing events. Nature 286, 779–783 (1980). https://doi.org/10.1038/286779a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/286779a0
This article is cited by
-
Conserved cryptic recombination signals in Vκ gene segments are cleaved in small pre-B cells
BMC Immunology (2009)
-
High-frequency deletion event ataprt locus of CHO cells: Detection and characterization of endpoints
Somatic Cell and Molecular Genetics (1989)
-
Immunoglobulin JH rearrangement in a T-cell line reflects fusion to the D H locus at a sequence lacking the nonamer recognition signal
Immunogenetics (1988)
-
Antibody diversity: New mechanism revealed
Nature (1986)
-
Recombination between an expressed immunoglobulin heavy-chain gene and a germline variable gene segment in a Ly 1+ B-cell lymphoma
Nature (1986)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
