Abstract
The use of established cell lines of various types has provided information on the properties of certain stem cells and on the conditions of their differentiation. No bone-forming cell line has so far been described. We report here the isolation, from in vitro differentiating teratocarcinoma cells1, of a line which, on subcutaneous injection into syngeneic mice, gives rise to ossicles. The appearance of ossicles is preceded by the formation of a cartilaginous matrix, thus reproducing what has been described as endochondral differentiation2. The ossicles obtained are rapidly colonized by host marrow, are non-malignant and stop growing 2 weeks after injection of the cells. In certain conditions, however, osteosarcomas, chondroosteosarcomas and fibrosarcomas can be obtained. From these tumours, cell lines have been isolated which retain in vitro the properties of the cells found in the tumour.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Nicolas, J. F., Avner, P., Gaillard, J., Guénet, J. L., Jakob, H. & Jacob, F. Cancer Res. 36, 4224–4231 (1976).
Pritchard, J. J. in Differentiation and Growth of Cells in Vertebrate Tissues (ed. Goldspink, G.) 101–128 (Chapman & Hall, London, 1974).
Bridges, J. B. Int. Rev. Cyt. 8, 253–278 (1959).
Wlodavski, K., Kobus, M. & Luczak, M. Nature 281, 386–387 (1979).
Reddi, A. H. & Anderson, W. A. J. Cell Biol. 69, 557–572 (1976).
Urist, M. R., Mikulski, A. & Lietze, A. Proc. natn. Acad. Sci. U.S.A. 70, 1828–1832 (1979).
Nicolas, J. F., Dubois, Ph., Jakob, H., Gaillard, J. & Jacob, F. Annls Microbiol. Inst. Pasteur, Paris 126 A, 3–22 (1975).
Friedenstein, A. J. & Chailakhyan, R. F. Transplantation 17, 331–340 (1974).
Cline, M. J. & Golde, D. W. Nature 277, 177–181 (1979).
Artzt, K. et al. Proc. natn. Acad. Sci. U.S.A. 70, 2988–2992 (1973).
Rothfels, K. H. & Siminovich, L. Stain Technol. 33, 73–77 (1958).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nicolas, J., Gaillard, J., Jakob, H. et al. Bone-forming cell line derived from embryonal carcinoma cells. Nature 286, 716–718 (1980). https://doi.org/10.1038/286716a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/286716a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.