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High-salt d(CpGpCpG), a left-handed Z′ DNA double helix

Abstract

The DNA tetramer d(CpGpCpG) or CGCG crystallizes from high-salt solution as a left-handed double helix, the Z′ helix. Its structure differs from that of the other known left-handed helix, Z-DNA, by a Cl′-exo sugar pucker at deoxyguanosines rather than C3′-endo, and these represent two alternative solutions to the same steric constraint arising from the syn glycosyl bond orientation. The apparent molecular basis for the Z to Z′ transition in going from intermediate to high salt is substitution of a bound anion for water at guanine amino groups, and consequent charge repulsion of anions and backbone phosphates.

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Drew, H., Takano, T., Tanaka, S. et al. High-salt d(CpGpCpG), a left-handed Z′ DNA double helix. Nature 286, 567–573 (1980). https://doi.org/10.1038/286567a0

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