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High-salt d(CpGpCpG), a left-handed Z′ DNA double helix

Nature volume 286, pages 567573 (07 August 1980) | Download Citation

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Abstract

The DNA tetramer d(CpGpCpG) or CGCG crystallizes from high-salt solution as a left-handed double helix, the Z′ helix. Its structure differs from that of the other known left-handed helix, Z-DNA, by a Cl′-exo sugar pucker at deoxyguanosines rather than C3′-endo, and these represent two alternative solutions to the same steric constraint arising from the syn glycosyl bond orientation. The apparent molecular basis for the Z to Z′ transition in going from intermediate to high salt is substitution of a bound anion for water at guanine amino groups, and consequent charge repulsion of anions and backbone phosphates.

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Author notes

    • Keiichi Itakura

    City of Hope National Medical Center, Duarte, California 91010

Affiliations

  1. Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125

    • Horace Drew
    • , Tsunehiro Takano
    • , Shoji Tanaka
    • , Keiichi Itakura
    •  & Richard E. Dickerson

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https://doi.org/10.1038/286567a0

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