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Expression of cloned β-endorphin gene sequences by Escherichia coli

Abstract

DNA coding for the opiate peptide β-endorphin has been cloned into bacterial plasmids in such a way as to direct the synthesis of a hybrid β-galactosidase/β-endorphin protein. This hybrid protein can readily be cleaved in vitro to release biologically active β-endorphin.

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Shine, J., Fettes, I., Lan, N. et al. Expression of cloned β-endorphin gene sequences by Escherichia coli. Nature 285, 456–461 (1980). https://doi.org/10.1038/285456a0

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