Abstract
We have used the bacterial plasmid pBR322 as a vehicle to isolate genes coding for selectable markers from higher eukaryotes. In this way, we have obtained the chicken thymidine kinase (tk) gene as a 2.2-kilobase EcoRI/HindIII insert in pBR322. The cloned gene transforms tk− animal cells with an efficiency equal to that of the cloned herpes simplex virus-1 tk gene.
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Perucho, M., Hanahan, D., Lipsich, L. et al. Isolation of the chicken thymidine kinase gene by plasmid rescue. Nature 285, 207–210 (1980). https://doi.org/10.1038/285207a0
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DOI: https://doi.org/10.1038/285207a0
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