Avian sarcoma virus-transforming protein, pp60src shows protein kinase activity specific for tyrosine

Abstract

The protein responsible for malignant transformation by avian sarcoma viruses (ASVs) has been identified as a phos-phoprotein of molecular weight 60,000 designated pp60src (refs 1–4). It has been suggested that this protein has a functional role in cellular transformation involving the phosphorylation of cellular proteins, for it was discovered that specific immunoprecipitates from ASV-transformed cells that contain pp60src catalysed the transfer of phosphate from [γ-32P]ATP to the heavy chain of rabbit immunoglobulin5,6. Additional studies involving the cell-free synthesis of the ASV src protein further demonstrated that the presence of the src polypeptide correlated with that presence of a phosphotransf erase activity7. Our studies, involving the biochemical purification of this protein, have demonstrated that the ASV-transforming gene product, pp60src, is itself a protein kinase. We have purified the pp60src protein approximately 5,000-fold using either conventional ion-exchange chromatography or immunoaffinity chromatography8,9. The resultant partially purified preparations contain a cyclic AMP-independent protein kinase activity8. We report here that the soluble phosphotransferase activity of partially purified pp60src results in the phosphorylation of exclusively tyrosine residues in a variety of proteins that serve as substrates.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Brugge, J. S. & Erikson, R. L. Nature 269, 346–348 (1977).

    ADS  CAS  Article  Google Scholar 

  2. 2

    Purchio, A. F., Erikson, E. & Erikson, R. L. Proc. natn. Acad. Sci. U.S.A. 74, 4661–4665 (1977).

    ADS  CAS  Article  Google Scholar 

  3. 3

    Purchio, A. F., Erikson, E., Brugge, J. S. & Erikson, R. L. Proc. natn. Acad. Sci. U.S.A. 75, 1567–1571 (1978).

    ADS  CAS  Article  Google Scholar 

  4. 4

    Brugge, J. S., Erikson, E., Collett, M. S. & Erikson, R. L. J. Virol. 26, 773–782 (1978).

    CAS  PubMed  PubMed Central  Google Scholar 

  5. 5

    Collett, M. S. & Erikson, R. L. Proc. natn. Acad. Sci. U.S.A. 75, 2021–2024 (1978).

    ADS  CAS  Article  Google Scholar 

  6. 6

    Levinson, A. D., Oppermann, H., Levintow, L., Varmus, H. E. & Bishop, J. M. Cell 15, 561–572 (1978).

    CAS  Article  Google Scholar 

  7. 7

    Erikson, E., Collett, M. S. & Erikson, R. L. Nature 274, 919–921 (1978).

    ADS  CAS  Article  Google Scholar 

  8. 8

    Erikson, R. L., Collett, M. S., Erikson, E. & Purchio, A. F. Proc. natn. Acad. Sci. U.S.A. 76, 6260–6264 (1979).

    ADS  CAS  Article  Google Scholar 

  9. 9

    Erikson, R. L., Collett, M. S., Erikson, E., Purchio, A. F. & Brugge, J. S. Cold Spring Harb. Symp. quant. Biol. 44 (in the press).

  10. 10

    Collett, M. S., Erikson, E. & Erikson, R. L. J. Virol. 29, 770–781 (1979).

    CAS  PubMed  PubMed Central  Google Scholar 

  11. 11

    Eckhart, W., Hutchinson, M. A. & Hunter, T. Cell 18, 925–933 (1979).

    CAS  Article  Google Scholar 

  12. 12

    Witte, O., Dasgupta, A. & Baltimore, D. Nature 283, 826–831 (1980).

    ADS  CAS  Article  Google Scholar 

  13. 13

    Hunter, T. & Sefton, B. W. Proc. natn. Acad. Sci. U.S.A. (in the press).

  14. 14

    Collett, M. S., Brugge, J. S. & Erikson, R. L. Cell 15, 1363–1369 (1978).

    CAS  Article  Google Scholar 

  15. 15

    Brugge, J. S. et al. J. Virol 29, 1196–1203 (1979).

    CAS  PubMed  PubMed Central  Google Scholar 

  16. 16

    Collett, M. S., Erikson, E., Purchio, A. F., Brugge, J. S. & Erikson, R. L. Proc. natn. Acad. Sci. U.S.A. 76, 3159–3163 (1979).

    ADS  CAS  Article  Google Scholar 

  17. 17

    Oppermann, H., Levinson, A. D., Varmus, H. E., Levintow, L. & Bishop, J. M. Proc. natn. Acad. Sci. U.S.A. 76, 1804–1808 (1979).

    ADS  CAS  Article  Google Scholar 

  18. 18

    Spudich, J. A. & Watts, S. J. biol. Chem. 246, 4866–4871 (1971).

    CAS  PubMed  Google Scholar 

  19. 19

    Hubbard, B. D. & Lazarides, E. J. Cell Biol. 80, 166–182 (1979).

    CAS  Article  Google Scholar 

  20. 20

    Shelanski, M. L., Gaskin, F. & Cantor, C. R. Proc. natn. Acad. Sci. U.S.A. 70, 765–768 (1973).

    ADS  CAS  Article  Google Scholar 

  21. 21

    Plimmer, R. H. A. Biochem. J. 35, 461–469 (1941).

    CAS  Article  Google Scholar 

  22. 22

    Mitchell, H. K. & Lunan, K. D. Archs Biochem. Biophys. 106, 219–222 (1964).

    CAS  Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Collett, M., Purchio, A. & Erikson, R. Avian sarcoma virus-transforming protein, pp60src shows protein kinase activity specific for tyrosine. Nature 285, 167–169 (1980). https://doi.org/10.1038/285167a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Sign up for the Nature Briefing newsletter for a daily update on COVID-19 science.
Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing