Retinoids elicit many biological and biochemical responses from cells in vitro1–5. One widely used criterion for the responsiveness of cells to retinoids is inhibition of growth; retinoids reduce the saturation density and/or growth rate of many normal and tumorigenic cell lines2,6,7. Propagation of eukaryotic cells has been demonstrated to be dependent on the presence of macromolecular growth factors such as epidermal growth factor (EGF)8–10, which can stimulate proliferation of epithelial and fibroblastic cell lines. We now describe the effect of retinoids on the binding of EGF to its receptor. Retinoic acid enhances binding of 125I-labelled EGF to various fibroblastic and epidermal cell lines. It has no marked effect on the affinity of this growth factor for its receptor, but increases the number of EGF receptor sites. Retinoic acid has little effect on the binding of concanavalin A (Con A) and insulin, indicating the specific nature of the action of retinoids on cell-surface glycoproteins. Treatment of cells with the phorbol ester 12-O-tetradecanoyl phorbol-13-acetate (TPA) and retinoic acid shows poor antagonism between these compounds on EGF binding. It has been previously shown that retinoids induce or stimulate differentiation of embryonal carcinoma cells11–13. EGF binding can be used as a marker to monitor differentiation of these cells.
Access optionsAccess options
Subscribe to Journal
Get full journal access for 1 year
only $3.90 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Rent or Buy article
Get time limited or full article access on ReadCube.
All prices are NET prices.
De Luca, L. M. Vitam. Horm. 35, 1–57 (1977).
Jetten, A. M., Jetten, M. E. R., Shapiro, S. & Poon, J. Expl Cell Res. 119, 289–299 (1979).
Hassell, J., Pennypacker, J., Kleinman, K., Pratt, R. & Yamada, K. Cell 17, 821–826 (1979).
Patt, L., Itaya, K. & Hakomori, S. Nature 273, 379–381 (1978).
Adamo, S., De Luca, L. M., Akalovsky, I. & Bhat, P. J. natn. Cancer Inst. 62, 1473–1478 (1979).
Lotan, R. Biochim. biophys. Acta (in the press).
De Luca, L. M. et al. Pure appl. Chem. 51, 581–591 (1979).
Todaro, G. J., De Larco, J. & Cohen, S. Nature 264, 26–31 (1976).
Carpenter, G. & Cohen, S. A. Rev. Biochem. 48, 193–216 (1979).
Gospodarowicz, D., Moran, J. & Mescher, A. in Molecular Control of Proliferation and Differentiation (eds Papaconstaniou, J. & Rutter, W. T.) 32–63 (Academic, New York, 1978).
Strickland, S. & Mahdavi, V. Cell 15, 393–404 (1978).
Jetten, A. M., Jetten, M. E. R. & Sherman, M. Expl Cell Res. 124, 381–391 (1979).
Jetten, A. M. & Jetten, M. E. R. Nature 278, 180–182 (1979).
Shoyab, M., De Larco, J. & Todaro, G. J. Nature 279, 387–391 (1979).
Pratt, R. & Pastan, I. Nature 272, 68–70 (1978).
Brown, K., Yeh, Y. & Holley, R. W. J. cell. Physiol. 100, 227–238 (1979).
Chytil, F. & Ong, D. in Receptors and Hormone Action Vol. II (eds O'Malley, B. W. & Birnbaumer, L.) 573–591 (Academic, New York, 1978).
Colburn, N., Former, B., Nelson, K. & Yuspa, S. Nature 282, 589–591 (1979).
Boutwell, R. & Verma, A. Pure appl. Chem. 51, 857–886 (1979).
Todaro, G. J., De Larco, J. & Sporn, M. Nature 276, 272–274 (1978).
Colburn, N. in Neoplastic Transformation in Differentiated Epithelial Cell Systems In Vitro (eds Franks, L. & Wigley, C.) 113–134 (Academic, New York, 1979).
Fisher, P., Flamm, M., Schachter, D. & Weinstein, I. Biochem. biophys. Res. Commun. 86, 1063–1068 (1979).
Rees, A., Adamson, E. & Graham, C. Nature 281, 309–311 (1979).
Sporn, M., Dunlop, N., Newton, D. & Smith, J. Fedn Proc. 35, 1332–1338 (1976).
Bollag, W. Eur. J. Cancer 8, 689–693 (1972).
Jetten, A. M. Ann. N. Y. Acad. Sci. (in the press).
About this article
The proliferative effects of retinoic acid on primary cultures of adult rat type II pneumocytes depend upon cell density
In Vitro Cellular & Developmental Biology - Animal (2010)
8-Cl-cAMP antagonizes mitogen-activated protein kinase activation and cell growth stimulation induced by epidermal growth factor
British Journal of Cancer (1999)
Altered distribution of the nuclear receptor rarβ accompanies proliferation and differentiation changes caused by retinoic acid in Caco-2 cells
In Vitro Cellular & Developmental Biology - Animal (1996)
Retinoic acid modulates epidermal growth factor receptor expression in human lung epithelial cancer cells
Journal of Biomedical Science (1995)
Regulation of human renal adenocarcinoma cell growth by retinoic acid and its interactions with epidermal growth factor
Kidney International (1994)