Abstract
Selectable plasmid vectors that contain a gene encoding resistance to the antibiotic methylenomycin A and that are suitable for the cloning of endonuclease-generated DNA fragments in Streptomyces species have been constructed and characterised.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Berdy, J. Adv. appl. Microbiol. 18, 309–406 (1974).
Hopwood, D. A. & Merrick, M. J. Bact. Rev. 41, 595–635 (1977).
Hopwood, D. A., Wright, H. M., Bibb, M. J. & Cohen, S. N. Nature 268, 171–174 (1977).
Baltz, R. H. J. gen. Microbiol. 107, 93–102 (1978).
Bibb, M. J., Ward, J. M. & Hopwood, D. A. Nature 274, 398–400 (1978).
Bibb, M. J., Freeman, R. F. & Hopwood, D. A. Molec. gen. Genet. 154, 155–166 (1977).
Schrempf, H. & Goebel, W. J. Bact. 131, 251–258 (1977).
Bibb, M. J. thesis, Univ. East Anglia (1978).
Bibb, M. J., Ward, J. M. & Hopwood, D. A. Devl Ind. Microbiol. (in the press).
Blin, N., Gabain, A. V. & Bujard, M. FEBS Lett. 53, 84–86 (1975).
Wright, L. F. & Hopwood, D. A. J. gen. Microbiol. 95, 96–106 (1976).
Hopwood, D. A. A. Rev. Microbiol. 32, 373–392 (1978).
Hopwood, D. A., Chater, K. F., Dowding, J. E. & Vivian, A. Bact. Rev. 37, 371–405 (1973).
Hopwood, D. A. & Wright, H. M. 2nd Int. Symp. Genetics of Industrial Microorganism (ed. Macdonald, K. D.) 607–619 (Academic, London, 1976).
Rigby, P. W. J., Dieckmann, M., Rhodes, C. & Berg, P. J. molec. Biol 113, 237–251 (1977).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
Willetts, N. S. J. Bact. 118, 778–782 (1974).
Meacock, P. A. & Cohen, S. N. Cell (in the press).
Wright, L. F. & Hopwood, D. A. J. gen. Microbiol. 96, 289–297 (1976).
Williams, W. K. & Katz, E. J. Antimicrob. Ag. Chemother. 11, 281–290 (1977).
Chang, A. C. Y. et al. Nature 275, 617–624 (1978).
Hopwood, D. A. & Chater, K. F. Phil. Trans. R. Soc. B (in the press).
Hopwood, D. A. Bact. Rev. 31, 373–403 (1967).
Wahl, G. M., Stern, M. & Stark, G. R. Proc. natn. Acad. Sci. U.S.A. 76, 3683–3687 (1979).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bibb, M., Schottel, J. & Cohen, S. A DNA cloning system for interspecies gene transfer in antibiotic-producing Streptomyces. Nature 284, 526–531 (1980). https://doi.org/10.1038/284526a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/284526a0
This article is cited by
-
Kinetics of growth and co-production of amylase and protease in novel marine actinomycete, Streptomyces lopnurensis KaM5
Folia Microbiologica (2021)
-
The tyrosine recombinase MrpA and its target sequence: a mutational analysis of the recombination site mrpS resulting in a new left element/right element (LE/RE) deletion system
Archives of Microbiology (2013)
-
Characterization of the tyrosine recombinase MrpA encoded by the Streptomyces coelicolor A3(2) plasmid SCP2*
Archives of Microbiology (2011)
-
Transformation ofStreptomyces lincolnensis protoplasts with plasmid vectors
Folia Microbiologica (1992)
-
Transposition of IS117 (the Streptomyces coelicolor A3(2) mini-circle) to and from a cloned target site and into secondary chromosomal sites
Molecular and General Genetics MGG (1990)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.