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Glucagon and glicentin immunoreactivity are topologically segregated in the α granule of the human pancreatic A cell


Glicentin is a 100-amino acid polypeptide purified from the porcine intestine1 and containing the immunodeterminants of glucagon2. We have previously reported3 the presence of glicentin immunoreactivity in the glucagon-containing, specific secretory granules (α granules) of the pancreatic and gastric A cell. With an improved immunocytochemical probe, the protein A–gold (pAg) technique4, we are now able to show that glucagon and glicentin-like material are topologically segregated in the a granule of the human pancreatic A cell.

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