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Are snRNPs involved in splicing?

Nature volume 283, pages 220–224 (1980)Cite this article

Abstract

Discrete, stable small RNA molecules are found in the nuclei of cells1 from a wide variety of eukaryotic organisms2. Many of these small nuclear RNA (snRNA) species, which range in size from about 90 to 220 nucleotides, have been well-characterised biochemically3–6, and some sequenced7,8. However, their function has remained obscure. The most abundant snRNA species exist as a closely related set of RNA–protein complexes called small nuclear ribonucleoproteins (snRNPs)9. snRNPs are the antigens recognised by antibodies from some patients with lupus erythematosus (LE), an autoimmune rheumatic disease10,11. Anti-RNP antibodies from lupus sera selectively precipitate snRNP species containing Ula7 and Ulb9 RNAs from mouse Ehrlich ascites cell nuclei, whereas anti-Sm antibodies bind these snRNPs and four others containing U2 (ref. 8), U4, US and U6 (ref. 9) RNAs. Both antibody systems precipitate the same seven prominent nuclear proteins (molecular weight 12,000–32,000). All molecules of the snRNAs U1, U2, U4, U5 and U6 appear to exist in the form of antigenic snRNPs9. The particles sediment at about 10S and each probably contains a single snRNA molecule. Indirect immunofluorescence studies (refs 12, 13, and unpublished observations) using anti-RNP and anti-Sm sera confirm the nuclear (but non-nucleolar) location of the antigenic snRNPs. Here we present several lines of evidence that suggest a direct involvement of snRNPs in the splicing of hnRNA. Most intriguing is the observation that the nucleotide sequence at the 5′ end of U1 RNA exhibits extensive complementarity to those across splice junctions in hnRNA molecules.

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References

  1. Weinberg, R. & Penman, S. J. molec. Biol. 38, 289–304 (1968).

    Article  CAS  Google Scholar 

  2. Hellung-Larsen, P. & Frederiksen, S. Comp. Biochem. Physiol. 58B, 273–281 (1977).

    CAS  Google Scholar 

  3. Ro-Choi, T. S. & Busch, H. in The Cell Nucleus (ed. Busch, H.) 151–208 (Academic, New York, 1974).

    Book  Google Scholar 

  4. Zieve, G. & Penman, S. Cell 8, 19–31 (1976).

    Article  CAS  Google Scholar 

  5. Benecke, B.-J. & Penman, S. Cell 12, 939–946 (1977).

    Article  CAS  Google Scholar 

  6. Jelinek, W. & Leinwand, L. Cell 15, 205–214 (1978).

    Article  CAS  Google Scholar 

  7. Reddy, R., Ro-Choi, T. S., Henning, D. & Busch, H. J. biol. Chem. 249, 6486–6494 (1974).

    CAS  PubMed  Google Scholar 

  8. Shibata, H. et al. J. biol. Chem. 250, 3909–3920 (1975).

    CAS  PubMed  Google Scholar 

  9. Lerner, M. R. & Steitz, J. A. Proc. natn. Acad. Sci. U.S.A. 76, 5495–5499 (1979).

    Article  ADS  CAS  Google Scholar 

  10. Notman, D. D., Kurata, N. & Tan, E. M. Ann. Int. Med. 83, 464–469 (1979).

    Article  Google Scholar 

  11. Provost, T. T. J. Invest. Dermatol. 72, 110–113 (1979).

  12. Mattioli, M. & Reichlin, M. J. Immun. 107, 1281–1290 (1971).

    CAS  PubMed  Google Scholar 

  13. Northway, J. O. & Tan, E. M. Clin. Immun. Immunopath. 1, 140–154 (1972).

    Article  CAS  Google Scholar 

  14. Weil, P. A., Luse, D. S., Segall, J. & Roeder, R. G. Cell 18, 469–484 (1979).

    Article  CAS  Google Scholar 

  15. Hamer, D. H. & Leder, P. Nature 281, 35–40 (1979).

    Article  ADS  CAS  Google Scholar 

  16. Mantei, N., Boll, W. & Weissmann, C. Nature 281, 40–46 (1979).

    Article  ADS  CAS  Google Scholar 

  17. Mulligan, R. C., Howard, B. H. & Berg, P. Nature 277, 108–114 (1979).

    Article  ADS  CAS  Google Scholar 

  18. Martin, T. et al. Cold Spring Harb. Symp. quant. Biol. 38, 921–932 (1973).

    Article  Google Scholar 

  19. Karn, J., Vidali, G., Boffa, L. C. & Allfrey, V. G. J. biol. Chem. 252, 7307–7322 (1977).

    CAS  PubMed  Google Scholar 

  20. Beyer, A. L., Christensen, M. E., Walker, B. W. & L. Stourgeon, W. M. Cell 11, 127–138 (1977).

    Article  CAS  Google Scholar 

  21. Howard, E. F. Biochemistry 17, 3228–3236 (1978).

    Article  CAS  Google Scholar 

  22. Deimel, B., Louis, C. & Sekeris, C. E. FEBS Lett. 73, 80–84 (1977).

    Article  CAS  Google Scholar 

  23. Northemann, W., Scheurlen, M., Gross, V. & Heinrich, P. C. Biochem. biophys. Res. Commun. 76, 1130–1137 (1977).

    Article  CAS  Google Scholar 

  24. Guimont-Ducamp, C., Sri-Widada, J. & Jeanteur, P. Biochimie 59, 755–758 (1977).

    Article  CAS  Google Scholar 

  25. Kim, S.-H. in Transfer RNA (ed. Altman S.) 248–293 (MIT, Cambridge, Mass., 1979).

    Google Scholar 

  26. Murray, V. & Holliday, R. FEBS Lett. 106, 5–7 (1979).

    Article  CAS  Google Scholar 

  27. Stark, B. C., Kole, R., Bowman, E. J. & Altman, S. Proc. natn. Acad. Sci. U.S.A. 75, 3717–3721 (1978).

    Article  ADS  CAS  Google Scholar 

  28. Blanchard, J.-M., Weber, J., Jelinek, W. & Darnell, J. E. Proc. natn. Acad. Sci. U.S.A. 75, 5344–5348 (1978).

    Article  ADS  CAS  Google Scholar 

  29. Manley, J. L., Sharp, P. A. & Gefter, M. L. J. molec. Biol. (in the press).

  30. Hamer, D. H., Smith, K. D., Boyer, S. H. & Leder, P. Cell 17, 725–735 (1979).

    Article  CAS  Google Scholar 

  31. Gruss, P., Lai, C.-J., Dhar, R. & Khoury, G. Proc. natn. Acad. U.S.A. 76, 4317–4321 (1979).

    Article  ADS  CAS  Google Scholar 

  32. Reanney, D. Nature 277, 598–600 (1979).

    Article  ADS  CAS  Google Scholar 

  33. Crick, F. Science 204, 264–271 (1979).

    Article  ADS  CAS  Google Scholar 

  34. Roberts, R. J. Nature 274, 530 (1978).

    Article  ADS  CAS  Google Scholar 

  35. Church, G. M., Slonimski, P. P. & Gilbert, W. Cell (in the press).

  36. Lomedico, P. et al. Cell 18, 545–558 (1979).

    Article  CAS  Google Scholar 

  37. Honjo, T. et al. Cell 18, 559–568 (1979).

    Article  CAS  Google Scholar 

  38. Sakano, H. et al. Nature 277, 627–633 (1979).

    Article  ADS  CAS  Google Scholar 

  39. Tonegawa, S., Maxam, A. M., Tizard, R., Bernard, O. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 75, 1485–1489 (1978).

    Article  ADS  CAS  Google Scholar 

  40. Bernard, O., Hozumi, N. & Tonegawa, S. Cell 15, 1133–1144 (1978).

    Article  CAS  Google Scholar 

  41. Max, E. E., Seidman, J. G. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 76, 3450–3454 (1979).

    Article  ADS  CAS  Google Scholar 

  42. Seidman, J. G., Max, E. E. & Leder, P. Nature 280, 370–375 (1979).

    Article  ADS  CAS  Google Scholar 

  43. van Ooyen, A., van den Berg, J., Mantei, N. & Weissmann, C. Science 206, 337–344 (1979).

    Article  ADS  CAS  Google Scholar 

  44. Lawn, R. M., Fritsch, E. F., Parker, R. C., Blake, G. & Maniatis, T. Cell 15, 1157–1174 (1978).

    Article  CAS  Google Scholar 

  45. Gannon, F. et al. Nature 278, 428–434 (1979).

    Article  ADS  CAS  Google Scholar 

  46. Catterall, J. F. et al. Nature 275, 510–513 (1978).

    Article  ADS  CAS  Google Scholar 

  47. Breathnach, R., Benoist, C., O'Hare, K., Gannon, F. & Chambon, P. Proc. natn. Acad. Sci. U.S.A. 75, 4853–4857 (1978).

    Article  ADS  CAS  Google Scholar 

  48. Ghosh, P. K., Reddy, V. R., Swinscoe, J., Lebowitz, P. & Weissman, S. M. J. molec. Biol. 126, 813–846 (1978).

    Article  CAS  Google Scholar 

  49. Fiers, W. et al. Nature 273, 113–120 (1978).

    Article  ADS  CAS  Google Scholar 

  50. Tsujimoto, Y. & Suzuki, Y. Cell 18, 591–600 (1979).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, 06510

    Michael R. Lerner, John A. Boyle, Stephen M. Mount, Sandra L. Wolin & Joan A. Steitz

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  1. Michael R. Lerner
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  2. John A. Boyle
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  4. Sandra L. Wolin
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  5. Joan A. Steitz
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Lerner, M., Boyle, J., Mount, S. et al. Are snRNPs involved in splicing?. Nature 283, 220–224 (1980). https://doi.org/10.1038/283220a0

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