Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Transposition protein of Tn3: identification and characterisation of an essential represser-controlled gene product

Abstract

Fusions that bring lac gene expression under the control of transcriptional and translational signals within the Tn3 element have been used to identify and characterise a Tn3-encoded ‘transposase’ (Tnp) peptide of MW 100,000 essential f or transposition. The gene specifying this product is regulated by the Tn3 repressor protein and is part of a bidirectional genetic unit that includes the repressor gene.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Campbell, A. et al. in DNA Insertion Elements, Plasmids and Episomes (eds Bukhari, A. I., Shapiro, J. A. & Adhya, S. L.) 15–22 (Cold Spring Harbor Laboratory, New York, 1977).

    Google Scholar 

  2. Heffron, F., Bedinger, P., Champoux, J. & Falkow, S. Proc. natn. Acad. Sci. U.S.A. 74, 702–706 (1977).

    Article  ADS  CAS  Google Scholar 

  3. Heffron, F., So, M. & McCarthy, B. J. Proc. natn. Acad. Sci. U.S.A. 75, 6012–6016 (1978).

    Article  ADS  CAS  Google Scholar 

  4. Kopecko, D. J. & Cohen, S. N. Proc. natn. Acad. Sci. U.S.A. 75, 1373–1377 (1975).

    Article  ADS  Google Scholar 

  5. Cohen, S. N., Casadaban, M. J., Chou, J. & Tu, C.-P. D. Cold Spring Harb. Symp. quant. Biol. 43, 1247–1255 (1979).

    Article  CAS  PubMed  Google Scholar 

  6. Casadaban, M. J., Chou, J. & Cohen, S. N. (in preparation).

  7. Chou, J., Casadaban, M. J., Lemaux, P. G. & Cohen, S. N. Proc. natn. Acad. Sci. U.S.A. 76, 4020–4024 (1979).

    Article  ADS  CAS  Google Scholar 

  8. Gill, R., Heffron, F., Dougan, G. & Falkow, S. J. Bact. 136, 742–756 (1978).

    CAS  PubMed  PubMed Central  Google Scholar 

  9. Adler, H. I., Fisher, W. D., Cohen, A. & Hardegree, A. A. Proc. natn. Acad. Sci. U.S.A. 57, 321–329 (1967).

    Article  ADS  CAS  Google Scholar 

  10. Sutcliffe, J. G. Proc. natn. Acad. Sci. U.S.A. 75, 3737–3741 (1978).

    Article  ADS  CAS  Google Scholar 

  11. Casadaban, M. J., Chou, J. & Cohen, S. N. (in preparation).

  12. Miller, J. H. in Experiments in Molecular Genetics,167–172 (Cold Spring Harbor Laboratory, New York, 1972).

    Google Scholar 

  13. Kretschmer, P. J., Chang, A. C. Y. & Cohen, S. N. J. Bact. 130, 888–899 (1977).

    CAS  PubMed  PubMed Central  Google Scholar 

  14. Kretschmer, P. J. & Cohen, S. N. J. Bact. 139, 515–519 (1979).

    CAS  PubMed  PubMed Central  Google Scholar 

  15. Sussenbach, J. S., Monfoort, C. H., Schiphof, R. & Stubberingh, E. E. Nucleic Acids Res. 3, 3193–3202 (1976).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Shine, J. & Dalgarno, L. Proc. natn. Acad. Sci. U.S.A. 71, 1342–1346 (1974).

    Article  ADS  CAS  Google Scholar 

  17. Pribnow, D. Proc. natn. Acad. Sci. U.S.A. 72, 784–788 (1975).

    Article  ADS  CAS  Google Scholar 

  18. Majors, J. Nature 256, 672–674 (1975).

    Article  ADS  CAS  PubMed  Google Scholar 

  19. Steege, D. A. Proc. natn. Acad. Sci. U.S.A. 74, 4163–4168 (1977).

    Article  ADS  CAS  Google Scholar 

  20. Gilbert, W. & Maxam, A. Proc. natn. Acad. Sci. U.S.A. 70, 3581–3585 (1973).

    Article  ADS  CAS  Google Scholar 

  21. Ptashne, M. et al. Science 194, 156–161 (1976).

    Article  ADS  CAS  PubMed  Google Scholar 

  22. Shapiro, J. Proc. natn. Acad. Sci. U.S.A. 76, 1933–1937 (1979).

    Article  ADS  CAS  Google Scholar 

  23. Meagher, R. B., Tait, R. C., Betlack, M. & Boyer, H. W. Cell 10, 521–536 (1977).

    Article  CAS  PubMed  Google Scholar 

  24. O'Farrell, P. H. J. biol. Chem. 250, 4007–4021 (1975).

    CAS  PubMed  Google Scholar 

  25. Pederson, S. et al. Molec. gen. Genet. 144, 339–344 (1976).

    Article  Google Scholar 

  26. Chang, A. C. Y. & Cohen, S. N. J. Bact. 134, 1141–1156 (1978).

    CAS  PubMed  PubMed Central  Google Scholar 

  27. Borck, K., Beggs, J., Brammar, W., Hopkins, A. & Murray, N. Molec. gen. Genet. 146, 199–207 (1976).

    Article  CAS  PubMed  Google Scholar 

  28. Dougan, G., Saul, M., Twigg, A., Gill, R. & Sherratt, D. J. Bact. 138, 48–54 (1979).

    CAS  PubMed  PubMed Central  Google Scholar 

  29. Timmis, K., Cabello, F. & Cohen, S. N. Molec. gen. Genet. 162, 121–137 (1978).

    Article  CAS  PubMed  Google Scholar 

  30. Cohen, S. N., Chang, A. C. Y. & Hsu, L. Proc. natn. Acad. Sci. U.S.A. 69, 2110–2114 (1972).

    Article  ADS  CAS  Google Scholar 

  31. Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).

    Article  ADS  CAS  Google Scholar 

  32. Neidhardt, F. C., Block, P. L. & Smith, D. F. J. Bact. 109, 736–747 (1974).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chou, J., Lemaux, P., Casadaban, M. et al. Transposition protein of Tn3: identification and characterisation of an essential represser-controlled gene product. Nature 282, 801–806 (1979). https://doi.org/10.1038/282801a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/282801a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing