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Molecular structure of a left-handed double helical DNA fragment at atomic resolution

Nature volume 282, pages 680–686 (1979)Cite this article

Abstract

The DNA fragment d(CpGpCpGpCpG) crystallises as a left-handed double helical molecule with Watson–Crick base pairs and an antiparallel organisation of the sugar phosphate chains. The helix has two nucleotides in the asymmetric unit and contains twelve base pairs per turn. It differs significantly from right-handed B-DNA.

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References

  1. Watson, J. D. & Crick, F. H. C. Nature 171, 737 (1953).

    Article  ADS  CAS  Google Scholar 

  2. Arnott, S., Chandrasekaran, R. & Selsing, E. Structure and Conformation of Nucleic Acids and Protein-Nucleic Acid Interactions (eds Sundaralingam, M. & Rao, S. T.) 477–603 (University Park Press, Baltimore, 1975).

    Google Scholar 

  3. Pohl, F. M. & Jovin, T. M. J. molec. Biol. 67, 375–396 (1972).

    Article  CAS  Google Scholar 

  4. de Rooij, J. F. M. thesis, Leiden Univ. (1979).

  5. Arentzen, R., van Boeckel, C. A. A., van der Marel, G. & van Boom, J. H. Synthesis 137 (1979).

  6. Kim, S. H. et al. J. molec. Biol. 75, 421–428 (1973).

    Article  CAS  Google Scholar 

  7. Blow, D. M. & Crick, F. H. C. Acta crystallogr. 12, 794–802 (1959).

    Article  CAS  Google Scholar 

  8. Blundell, T. L. & Johnson, L. N. Protein Crystallography (Academic, London, 1976).

    Google Scholar 

  9. Hendrickson, W. A. & Konnert, J. Biomolecular Structure, Conformation, Function and Evolution Vol. 1 (ed. Srinivasan, R.) 43–57 (Pergamon, Oxford, 1979).

    Google Scholar 

  10. Arnott, S., Smith, P. J. C. & Chandrasekaran, R., CRC Handbook of Biochemistry and Molecular Biology 3rd edn Vol. II (ed. Fasman, G. D.) (CRC, Cleveland, 1976).

    Google Scholar 

  11. Rosenberg, J. M. et al. Nature 243, 150–154 (1973).

    Article  ADS  CAS  Google Scholar 

  12. Day, R. O., Seeman, N. C., Rosenberg, J. M. & Rich, A. Proc. natn. Acad. Sci. U.S.A. 70, 849–853 (1973).

    Article  ADS  CAS  Google Scholar 

  13. Viswamitra, M. A. et al. Nature 273, 687–690 (1978).

    Article  ADS  CAS  Google Scholar 

  14. Quigley, G. J. & Rich, A. Science 194, 796–806 (1976).

    Article  ADS  CAS  Google Scholar 

  15. Jack, A., Ladner, J. E., Rhodes, D., Brown, R. S. & Klug, A. J. molec. Biol. 111, 315–328 (1977).

    Article  CAS  Google Scholar 

  16. Holbrook, S. R., Sussman, J. L., Warrant, R. W., Church, G. M. & Kim, S. H. Nucleic Acids Res. 4, 2811–2830 (1977).

    Article  CAS  Google Scholar 

  17. Stout, C., Mizano, J., Rubin, J., Brenner, T., Rao, S. T. & Sundaralingam, M. Nucleic Acids Res. 3, 1111–1123 (1976).

    Article  CAS  Google Scholar 

  18. Wang, A. H.-J., Nathans, J., van der Marel, G., van Boom, J. H. & Rich, A. Nature 276, 471–474 (1978).

    Article  ADS  CAS  Google Scholar 

  19. Rodley, G. A., Scobie, R. S., Bates, R. H. T. & Levitt, R. M. Proc. natn. Acad. Sci. U.S.A 73, 2959–2963 (1976).

    Article  ADS  CAS  Google Scholar 

  20. Sasisekharan, V., Pattabiraman, N. & Gupta, G. Proc. natn. Acad. Sci. U.S.A. 75, 4092–4096 (1978).

    Article  ADS  CAS  Google Scholar 

  21. Pohl, F. M. Nature 260, 365–366 (1976).

    Article  ADS  CAS  Google Scholar 

  22. Gray, D. M., Edmondson, S. P., Lang, D., Vaughan, M. & Nave, C. Nucleic Acids Res. 6, 2089–2107 (1979).

    Article  CAS  Google Scholar 

  23. Pohl, F. M., Jovin, T. M., Baehr, W. & Holbrook, J. J. Proc. natn. Acad. Sci. U.S.A. 9, 3805–3809 (1972).

    Article  ADS  Google Scholar 

  24. Patel, D. J., Canuel, L. L. & Pohl, F. M. Proc. natn. Acad. Sci. U.S.A. 76, 2508–2511 (1979).

    Article  ADS  CAS  Google Scholar 

  25. Arnott, S., Chandrasekaran, R., Hukins, D. W. L., Smith, P. J. C. & Watts, L. J. molec. Biol. 88, 523–533 (1974).

    Article  CAS  Google Scholar 

  26. Mitsui, Y. et al. Nature 228, 1166–1169 (1970).

    Article  ADS  CAS  Google Scholar 

  27. Weissbach, A. & Lisio, A. Biochemistry 4, 196–200 (1965).

    Article  CAS  Google Scholar 

  28. Mercado, C. M. & Tomasz, M. Biochemistry 16, 2040–2046 (1977).

    Article  CAS  Google Scholar 

  29. Zimmer, Ch. & Luck, G. Biochim. biophys. Actu 361, 11–32 (1974).

    Article  CAS  Google Scholar 

  30. Stettler, V. H., Weber, H., Koller, T. & Weissmann, C. J. molec. Biol. 131, 21–40 (1979).

    Article  CAS  Google Scholar 

  31. Levitt, M. & Warshel, A. J. Am. chem. Soc. 100, 2607–2613 (1978).

    Article  CAS  Google Scholar 

  32. Rich, A., Quigley, G. J. & Wang, A. H.-J. in Stereodynamics of Molecular Systems (ed.Sarma, R. H.) 315–330 (Pergamon, New York, 1979).

    Book  Google Scholar 

  33. Gray, D. M., Tinoco, I. Jr & Chamberlin, M. J. Biopolymers 11, 1235–1258 (1972).

    Article  CAS  Google Scholar 

  34. Patel, D. in Stereodynamics of Molecular Systems (ed. Sarma, R. H.) 397–422 (Pergamon, New York, 1979).

    Book  Google Scholar 

  35. Astell, C. R., Smith, M., Chow, M. B. & Ward, D. C. Cell 17, 691–703 (1979).

    Article  CAS  Google Scholar 

  36. Isono, K. & Yourno, J. Proc. natn. Acad. Sci. U.S.A. 71, 1612–1617 (1974).

    Article  ADS  CAS  Google Scholar 

  37. Searle, C. E. (ed.) Chemical Carcinogens (American Chemical Society, Washington D.C., 1978).

  38. Bird, A. P., Taggart, M. H. & Smith, B. A. Cell 17, 889–901 (1979).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139

    Andrew H.-J. Wang, Gary J. Quigley, Francis J. Kolpak, James L. Crawford & Alexander Rich

  2. Department of Organic Chemistry, Gorlaeus Laboratories, University of Leiden, The Netherlands

    Jacques H. van Boom & Gijs van der Marel

Authors
  1. Andrew H.-J. Wang
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  2. Gary J. Quigley
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  3. Francis J. Kolpak
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  4. James L. Crawford
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  5. Jacques H. van Boom
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  6. Gijs van der Marel
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  7. Alexander Rich
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Wang, AJ., Quigley, G., Kolpak, F. et al. Molecular structure of a left-handed double helical DNA fragment at atomic resolution. Nature 282, 680–686 (1979). https://doi.org/10.1038/282680a0

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