Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone

Nature volume 281, pages 544–548 (1979)Cite this article

Abstract

DNA coding for human growth hormone was constructed by using chemically synthesised DNA in conjunction with enzymatically prepared cDNA. This ‘hybrid’ gene was expressed in Escherichia coli under the control of the lac promoter. A polypeptide was produced having the size and immunological properties characteristic of mature human growth hormone.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Raben, M. S. J. clin. Endocr. 18, 901–904 (1958).

    Article  CAS  Google Scholar 

  2. Proc. of NIAMDD Symp. Publ. No. 74–612 (ed. Raiti, S.) (Baltimore, Maryland, 1973).

  3. Bancroft, F. C. Expl Cell Res. 79, 275–278 (1973).

    Article  CAS  Google Scholar 

  4. Seeburg, P. H., Shine, J., Martial, J. A., Baxter, J. D. & Goodman, H. M. Nature 270, 486–494 (1977).

    Article  ADS  CAS  Google Scholar 

  5. Goodman, H. M. et al. in Specific Eukaryotic Genes (eds Engberg, J., Klenow, H. & Leick, V.) 179–190 (Munskagaard, Copenhagen, 1979).

    Google Scholar 

  6. Villa-Komaroff, L. et al. Proc. natn. Acad. Sci. U.S.A. 75, 3727–3731 (1978).

    Article  ADS  CAS  Google Scholar 

  7. Seeburg, P. H. et al. Nature 276, 795–798 (1978).

    Article  ADS  CAS  Google Scholar 

  8. Mercereau-Puijalon, O. et al. Nature 275, 505–510 (1978).

    Article  ADS  CAS  Google Scholar 

  9. Fraser, T. H. & Bruce, B. J. Proc. natn. Acad. Sci. U.S.A. 75, 5936–5940 (1978).

    Article  ADS  CAS  Google Scholar 

  10. Chang, A. C. Y. et al. Nature 275, 617–624 (1978).

    Article  ADS  CAS  Google Scholar 

  11. Itakura, K. et al. Science 198, 1056–1063 (1977).

    Article  ADS  CAS  Google Scholar 

  12. Goeddel, D. V. et al. Proc. natn. Acad. Sci. U.S.A. 76, 106–110 (1979).

    Article  ADS  CAS  Google Scholar 

  13. Crea, R., Kraszewski, A., Hirose, T. & Itakura, K. Proc. natn. Acad. Sci. U.S.A. 75, 5765–5769 (1978).

    Article  ADS  CAS  Google Scholar 

  14. Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1978).

    Article  ADS  Google Scholar 

  15. Bolivar, F. et al. Gene 2, 95–113 (1977).

    Article  CAS  Google Scholar 

  16. Backman, K., Ptashne, M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 73, 4174–4178 (1976).

    Article  ADS  CAS  Google Scholar 

  17. Ulrich, A. et al. Science 196, 1313–1319 (1977).

    Article  ADS  Google Scholar 

  18. Wickens, M. P., Buell, G. N. & Schimke, R. T. J. biol. Chem. 253, 2483–2495 (1978).

    CAS  PubMed  Google Scholar 

  19. Klenow, H. & Henningsen, I. Proc. natn. Acad. Sci. U.S.A. 65, 168–171 (1970).

    Article  ADS  CAS  Google Scholar 

  20. Curtiss, R. III et al. in Molecular Cloning of Recombinant DNA (eds Scott, W. A. & Werner, R.) 99–111 (Academic, New York, 1977).

    Google Scholar 

  21. Grunstein, M. & Hogness, D. S. Proc. natn. Acad. Sci. U.S.A. 72, 3961–3965 (1975).

    Article  ADS  CAS  Google Scholar 

  22. Shine, J., Seeburg, P. H., Martial, J. A., Baxter, J. D. & Goodman, H. M. Nature 270, 494–499 (1977).

    Article  ADS  CAS  Google Scholar 

  23. Li, C. H. Proc. Am. phil. Soc. 116, 365 (1972).

    CAS  Google Scholar 

  24. Backman, K. & Ptashne, M. Cell 13, 65–71 (1978).

    Article  CAS  Google Scholar 

  25. Boyer, H. W. et al. in Recombinant Molecules: Impact on Science and Society, (eds Been, R. F. & Bassett, E. G.) 9–20 (Raven, New York, 1977).

    Google Scholar 

  26. Maizels, N. Nature 249, 647 (1974).

    Article  ADS  CAS  Google Scholar 

  27. Mauer, R. thesis, Harvard Univ. (1978).

  28. Maizel, J. V. Jr Meth. Virol. 5, 180–246 (1971).

    Google Scholar 

  29. Roberts, J. & Roberts, C. Proc. natn. Acad. Sci. U.S.A. 72, 147–151 (1975).

    Article  ADS  CAS  Google Scholar 

  30. Katz, L., Kingsbury, D. T. & Helinski, D. R. J. Bact. 144, 577–591 (1973).

    Article  Google Scholar 

  31. Kessler, S. W. J. Immun. 177, 1482–1490 (1976).

    Google Scholar 

  32. Roberts, T. M., Kacich, R. & Ptashne, M. Proc. natn. Acad. Sci. U.S.A. 76, 760–764 (1979).

    Article  ADS  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Molecular Biology, Genentech Inc., 460 Point San Bruno Boulevard, South San Francisco, California, 94080

    David V. Goeddel, Herbert L. Heyneker, Daniel G. Yansura, Michael J. Ross, Giuseppe Miozzari & Peter H. Seeburg

  2. Division of Organic Chemistry, Genentech Inc., 460 Point San Bruno Boulevard, South San Francisco, California, 94080

    Roberto Crea

  3. Division of Biology, City of Hope National Medical Center, Duarte, California, 91010

    Toyohara Hozumi, Rene Arentzen & Keiichi Itakura

Authors
  1. David V. Goeddel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Herbert L. Heyneker
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Toyohara Hozumi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Rene Arentzen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Keiichi Itakura
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Daniel G. Yansura
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Michael J. Ross
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Giuseppe Miozzari
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Roberto Crea
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Peter H. Seeburg
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Goeddel, D., Heyneker, H., Hozumi, T. et al. Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone. Nature 281, 544–548 (1979). https://doi.org/10.1038/281544a0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/281544a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing