Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Membrane crystals of ubiquinone:cytochrome c reductase from Neurospora mitochondria

Abstract

HIGH-RESOLUTION X-ray diffraction or electron microscopy studies of membrane proteins are hampered by the difficulty in obtaining crystals or periodic aggregates of the proteins. So far, only two examples of membrane proteins forming highly ordered arrays are known, the purple membrane of Halobacterium halobium1 and a membranous preparation of cytochrome c oxidase from beef heart2. Ubiquinone:cytochrome c reductase, a multiprotein enzyme, contributes about 10% of the inner mitochondrial membrane protein3. The enzyme is isolated as a dimer (molecular weight (MW) 550,000) and the monomeric unit comprises two b cytochromes (MW 30,000 each), a cytochrome c1 (MW 31,000), an iron-sulphur subunit (MW 25,000) and six subunits without known prosthetic groups (MWs 50,000, 45,000, 45,000, 14,000, 12,000 and 8,000)3,4. The enzyme catalyses the reduction of ferricytochrome c by reduced ubiquinone and conserves the energy generated by the oxidation-reduction reaction by translocating protons across the inner mitochondrial membrane5. Cytochrome c reductase is also of interest with respect to the genetics and biogenesis of mitochondria. The cytochrome b subunits of this enzyme belong to the small group of proteins which are translated on mitochondrial ribosomes6 and coded for by mitochondrial DNA7. We report here a method for preparing membrane crystals of ubiquinone:cytochrome c reductase isolated from Neurospora crassa.

This is a preview of subscription content, access via your institution

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

All prices are NET prices.

References

  1. Unwin, P. N. T. & Henderson, R. J. molec. Biol. 94, 425–440 (1975).

    Article  CAS  Google Scholar 

  2. Henderson, R., Capaldi, R. A. & Leigh, J. S. J. molec. Biol. 112, 631–648 (1977).

    Article  CAS  Google Scholar 

  3. Rieske, J. S. Biochim. biophys. Acta 456, 195–247 (1976).

    Article  CAS  Google Scholar 

  4. Weiss, H. & Kolb, H. J. Eur. J. Biochem. (in the press).

  5. Mitchell, P. FEBS Lett. 43, 189–194 (1974).

    Article  CAS  Google Scholar 

  6. Weiss, H. & Ziganke, B. Eur. J. Biochem. 41, 63–71 (1974).

    Article  CAS  Google Scholar 

  7. Tzagoloflf, A., Akai, A., Needleman, R. B. & Zulch, G. J. biol. Chem. 250, 8236–8242 (1975).

    Google Scholar 

  8. Holloway, P. S. Analyt. Biochem. 53, 304–308 (1973).

    Article  CAS  Google Scholar 

  9. Erickson, H. P., Voter, W. A. & Leonard, K. Meth. Enzym. 49, 39–63 (1978).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

WINGFIELD, P., ARAD, T., LEONARD, K. et al. Membrane crystals of ubiquinone:cytochrome c reductase from Neurospora mitochondria. Nature 280, 696–697 (1979). https://doi.org/10.1038/280696a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/280696a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing