Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Seminalplasmin—an antimicrobial protein from bovine seminal plasma which acts in E. coli by specific inhibition of rRNA synthesis

Abstract

SEMINAL PLASMA—the fluid in which spermatozoa are ejaculated—has been known to inhibit reversibly both RNA and protein synthesis in sperm cells1–3. Here we report the isolation from seminal plasma, purification to homogeneity, and characterisation of a protein which we have called ‘seminalplasmin’, that specifically inhibits rRNA synthesis in Escherichia coli and is highly antimicrobial.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Bhargava, P. M. Nature 179, 1120–1121 (1957).

    ADS  CAS  Article  Google Scholar 

  2. 2

    Bhargava, P. M., Bishop, M. W. H. & Work, T. S. Biochem. J. 73, 247–256 (1959).

    CAS  Article  Google Scholar 

  3. 3

    Abraham, K. A. & Bhargava, P. M. Biochem. J. 86, 298–307 (1963).

    CAS  Article  Google Scholar 

  4. 4

    Premkumar, E. & Bhargava, P. M. Nature new Biol. 240, 139–143 (1972).

    CAS  Article  Google Scholar 

  5. 5

    Premkumar, E. & Bhargava, P. M. Indian J. Biochem. Biophys. 10, 239–253 (1973).

    CAS  PubMed  Google Scholar 

  6. 6

    Reddy, E. S. P., Sitaram, N., Bhargava, P. M. & Scheit, K. H. J. molec. Biol. (submitted).

  7. 7

    Reisfield, R. A., Lewis, U. J. & Williams, D. E. Nature 195, 281–283 (1962).

    ADS  Article  Google Scholar 

  8. 8

    Techniques for High Resolution Electrophoresis (ORTEC Application Note AN 32A) (ORTEC Inc., Oak Ridge, Tennessee, 1973).

  9. 9

    Laemmli, U. K. Nature 227, 680–685 (1970).

    ADS  CAS  Article  Google Scholar 

  10. 10

    Winter, A. & Karlsson, C. Preparative Electrofocusing in Density Gradient (LKB Application Note 219) (LKB Produkter, Bomma, Sweden, 1976).

    Google Scholar 

  11. 11

    Scheit, K. H., Reddy, E. S. P. & Bhargava, P. M. Nature 279, 728–731 (1979).

    ADS  CAS  Article  Google Scholar 

  12. 12

    Morgan, E. A., Ikemura, T., Lindahl, L., Fallon, A. M. & Nomura, M. Cell 13, 335–344 (1978).

    CAS  Article  Google Scholar 

  13. 13

    Lizzarini, R. A. & Santangeele, E. J. Bact. 95, 1212–1220 (1968).

    Google Scholar 

  14. 14

    Weiss, J., Elsbach, P., Olsson, I. & Odeberg, H. J. biol. Chem. 253, 2664–2672 (1978).

    CAS  PubMed  Google Scholar 

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

REDDY, E., BHARGAVA, P. Seminalplasmin—an antimicrobial protein from bovine seminal plasma which acts in E. coli by specific inhibition of rRNA synthesis. Nature 279, 725–728 (1979). https://doi.org/10.1038/279725a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing