Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Tn554—a site-specific represser-controlled transposon in Staphylococcus aureus

Abstract

PREVIOUS studies have defined a specific element in Staphylococcus aureus containing determinants of inducible erythromycin resistance and spectinomycin resistance. This element has many plasmid-like properties1,2 but is not associated with detectable extra-chromosomal DNA2. The resistances are similar in mechanism to those ordinarily associated with plasmids3,4 (J. Davies, personal communication); the element can be transferred by transduction2 or transformation to a rec recipient with the same efficiency as to a rec+, and the two determinants show essentially 100% linkage in genetic transfer. Consequently, this linkage group has previously been referred to as a ‘pseudoplasmid’ (ref. 2). On the basis of the results presented here, we suggest that this pseudoplasmid is actually the prototype of a new class of transposon, which functions by means of a highly efficient, represser-controlled, site-specific integration–excision mechanism. This transposon, designated Tn554 (EmSp), can be most easily envisaged as a prophage-like element that lacks replicative autonomy and other vegetative phage functions.

Your institute does not have access to this article

Relevant articles

Open Access articles citing this article.

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

All prices are NET prices.

References

  1. Novick, R., Wyman, L., Bouanchaud, D. & Murphy, E. in Microbiology-1974 (ed. Schlessinger, D.) 115–129 (American Society for Microbiology, Washington, D.C., 1975).

    Google Scholar 

  2. Wyman, L., Goering, R. V. & Novick, R. P. Genetics 76, 681–702 (1974).

    CAS  PubMed  PubMed Central  Google Scholar 

  3. Lai, C. J. & Weisblum, B. Proc. natn. Acad. Sci. U.S.A. 68, 856–860 (1971).

    ADS  CAS  Article  Google Scholar 

  4. Weisblum, B., Siddhikol, C., Lai, C. J. & Demohn, V. J. Bact. 106, 835–847 (1971).

    CAS  PubMed  Google Scholar 

  5. Novick, R. P. J. gen. Microbiol. 33, 121–136 (1963).

    CAS  Article  Google Scholar 

  6. Pattee, P. A. & Neveln, D. S. J. Bact. 124, 201–211 (1975).

    CAS  PubMed  Google Scholar 

  7. Novick, R. P. et al. Plasmid 2, 109–129 (1979).

    CAS  Article  Google Scholar 

  8. Pattee, P. A., Thompson, N. E., Haubrich, D. & Novick, R. P. Plasmid 1, 38–51 (1977).

    CAS  Article  Google Scholar 

  9. Pattee, P. A. et al. Abstr. 78th A. Mtng Am. Soc. Microbiol. (1978).

  10. Kleckner, N. Cell 11, 11–23 (1977).

    CAS  Article  Google Scholar 

  11. Pattee, P. A., Kloos, W. E., Bodensteiner, J. B. & Zara, A. J. Virol. 2, 652–654 (1968).

    CAS  PubMed  PubMed Central  Google Scholar 

  12. Campbell, A. M. Adv. Genet. 11, 101–145 (1962).

    Article  Google Scholar 

  13. Novick, R. P. Molec. gen. Genet. 135, 131–147 (1974).

    CAS  Article  Google Scholar 

  14. MacHattie, L. A. & Jackowski, J. B. in DNA Insertion Elements, Plasmids, and Epitomes (eds Bukhari, A. I., Shapiro, J. A. & Adhya, S. L.) 219–228 (Cold Spring Harbor Laboratory, New York, 1977).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

PHILLIPS, S., NOVICK, R. Tn554—a site-specific represser-controlled transposon in Staphylococcus aureus. Nature 278, 476–478 (1979). https://doi.org/10.1038/278476a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/278476a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing