Abstract
PREVIOUS studies have defined a specific element in Staphylococcus aureus containing determinants of inducible erythromycin resistance and spectinomycin resistance. This element has many plasmid-like properties1,2 but is not associated with detectable extra-chromosomal DNA2. The resistances are similar in mechanism to those ordinarily associated with plasmids3,4 (J. Davies, personal communication); the element can be transferred by transduction2 or transformation to a rec− recipient with the same efficiency as to a rec+, and the two determinants show essentially 100% linkage in genetic transfer. Consequently, this linkage group has previously been referred to as a ‘pseudoplasmid’ (ref. 2). On the basis of the results presented here, we suggest that this pseudoplasmid is actually the prototype of a new class of transposon, which functions by means of a highly efficient, represser-controlled, site-specific integration–excision mechanism. This transposon, designated Tn554 (EmSp), can be most easily envisaged as a prophage-like element that lacks replicative autonomy and other vegetative phage functions.
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References
Novick, R., Wyman, L., Bouanchaud, D. & Murphy, E. in Microbiology-1974 (ed. Schlessinger, D.) 115–129 (American Society for Microbiology, Washington, D.C., 1975).
Wyman, L., Goering, R. V. & Novick, R. P. Genetics 76, 681–702 (1974).
Lai, C. J. & Weisblum, B. Proc. natn. Acad. Sci. U.S.A. 68, 856–860 (1971).
Weisblum, B., Siddhikol, C., Lai, C. J. & Demohn, V. J. Bact. 106, 835–847 (1971).
Novick, R. P. J. gen. Microbiol. 33, 121–136 (1963).
Pattee, P. A. & Neveln, D. S. J. Bact. 124, 201–211 (1975).
Novick, R. P. et al. Plasmid 2, 109–129 (1979).
Pattee, P. A., Thompson, N. E., Haubrich, D. & Novick, R. P. Plasmid 1, 38–51 (1977).
Pattee, P. A. et al. Abstr. 78th A. Mtng Am. Soc. Microbiol. (1978).
Kleckner, N. Cell 11, 11–23 (1977).
Pattee, P. A., Kloos, W. E., Bodensteiner, J. B. & Zara, A. J. Virol. 2, 652–654 (1968).
Campbell, A. M. Adv. Genet. 11, 101–145 (1962).
Novick, R. P. Molec. gen. Genet. 135, 131–147 (1974).
MacHattie, L. A. & Jackowski, J. B. in DNA Insertion Elements, Plasmids, and Epitomes (eds Bukhari, A. I., Shapiro, J. A. & Adhya, S. L.) 219–228 (Cold Spring Harbor Laboratory, New York, 1977).
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PHILLIPS, S., NOVICK, R. Tn554—a site-specific represser-controlled transposon in Staphylococcus aureus. Nature 278, 476–478 (1979). https://doi.org/10.1038/278476a0
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DOI: https://doi.org/10.1038/278476a0
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