Bio-selective membrane electrode using tissue slices


MEMBRANE electrodes based on living bacterial cells1 as biocatalysts have been shown to be useful sensors for amino acids and other biomolecules. We report here that we have now developed a highly effective membrane electrode for glutamine using slices of porcine kidney tissue as the biocatalytic layer. This tissue-based electrode yields excellent selectivity and sensitivity while having the additional advantages of simplicity and low cost. Further, the tissue-based electrode shows a much longer useful lifetime than comparable electrodes prepared with the isolated porcine enzyme.

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RECHNITZ, G., ARNOLD, M. & MEYERHOFF, M. Bio-selective membrane electrode using tissue slices. Nature 278, 466–467 (1979).

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