Bio-selective membrane electrode using tissue slices

Abstract

MEMBRANE electrodes based on living bacterial cells¹ as biocatalysts have been shown to be useful sensors for amino acids and other biomolecules. We report here that we have now developed a highly effective membrane electrode for glutamine using slices of porcine kidney tissue as the biocatalytic layer. This tissue-based electrode yields excellent selectivity and sensitivity while having the additional advantages of simplicity and low cost. Further, the tissue-based electrode shows a much longer useful lifetime than comparable electrodes prepared with the isolated porcine enzyme.