Demonstration of MHC-specific haemolytic plaque-forming cells

Abstract

IT would be convenient to be able to measure the antibody response to alloantigens of the major histocompatibility complex (MHC) at a cellular level. As MHC alloantigens are expressed on erythrocyte membranes in mice one might suppose that a conventional Jerne plaque assay with enumeration of haemolytic plaque-forming cells (allo-PFC) against a lawn of suitable allogeneic erythrocytes would be straightforward. In fact such assays have proved difficult, with very few plaques being detected1,2. We have found the same problem in detecting allo-PFC in the spleens of alloimmunised rats. Although alloantisera raised in appropriate strain combinations have a high titre in a direct complement dependent haemolytic assay (Fig. 1) emphasising the potential value of this convenient assay for alloantibody in the rat3,4, very few allo-PFC were found in spleen cell suspensions from hyperimmunised donors. We show here that the discrepancy may be due to the homogeneity of the antibody product of a single PFC compared with the heterogeneity of serum antibody.

References

  1. 1

    Hildemann, W. H. & Pinkerton, W. J. J. exp. Med. 124, 885–900 (1966).

  2. 2

    Hoecker, G., Ramos, A. & Ferreira, A. Transplantn Proc. 2, 76–82 (1970).

  3. 3

    Jensenius, J. Transplantation 22, 415–419 (1976).

  4. 4

    Galfré, G. et al. Nature 266, 550–552 (1977).

  5. 5

    Howard, J. C., Butcher, G. W., Galfré, G. & Milstein, C. Curr. Topics Microbiol. Immun. 81, 54–60 (1978).

  6. 6

    Cunningham, A. J. & Szenberg, A. Immunology 14, 599–600 (1968).

  7. 7

    Hart, D. N. J. & Fabre, J. W. Transplantation (in the press).

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HOWARD, J., CORVALAN, J. Demonstration of MHC-specific haemolytic plaque-forming cells. Nature 278, 449–451 (1979). https://doi.org/10.1038/278449a0

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