Dopamine inhibits adenylate cyclase in human prolactin-secreting pituitary adenomas

Abstract

THERE is evidence for the existence of multiple forms of dopamine (DA) receptors1–3. In particular, some of these receptors are coupled to adenylate cyclase (DA-stimulating enzyme activity), whereas others seem to be independent3–5. These two classes of receptors, which have been designated D-1 and D-2, respectively, would also have different pharmacological properties3. Pituitary mammotroph DA receptors regulating prolactin (PRL) secretion are considered to be typical D-2 receptors. A DA-stimulated adenylate cyclase has not been detected in normal anterior pituitaries3,5,6; furthermore, several studies indicate that cyclic AMP is stimulatory and not inhibitory to PRL secretion7,8. We report here that in homogenates of human PRL adenomas, in which dopaminergic agonists act as inhibitors of PRL secretion, basal adenylate cyclase is inhibited by DA. The DA receptors mediating this inhibition have the same pharmacological properties as those regulating PRL secretion.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Cools, A. R. & Van Rossum, J. M. Psychopharmacologia 45, 243–254 (1976).

  2. 2

    Creese, J., Burt, D. R. & Snyder, S. H. Life Sci. 17, 993–1002 (1975).

  3. 3

    Kebabian, J. W. & Calne, D. B. Nature 277, 93–96 (1979).

  4. 4

    Kebabian, J. W., Petzold, G. L. & Greengard, P. Proc. natn. Acad. Sci. U.S.A 69, 2145–2149 (1972).

  5. 5

    Spano, P. F., Govoni, S. & Trabucchi, M. Adv. Biochem. Psychopharmac. 19, 155–166 (1977).

  6. 6

    Schmidt, M. G. & Hill, L. E. Life Sci. 20, 789–797 (1977).

  7. 7

    Hill, M. K., MacLeod, R. M. & Orcutt, P. Endocrinology 99, 1612–1617 (1976).

  8. 8

    Labrie, F. et al. in Advances in Cyclic Nucleotide Res. Vol. 5 (eds Drummond, G. I.,Geengard, P. & Robison, G. A.) 787–801 (Raven, New York, 1975).

  9. 9

    Maizel, J. V. Jr in Methods in Virology, Vol 5 (eds Maramorosch, K. & Koprowski, H.) 179–244 (Academic, New York, 1971).

  10. 10

    Fluckiger, E. et al. Experientia 34, 1330–1332 (1978).

  11. 11

    Calabro, M. A. & MacLeod, R. M. Neuroendocrinology 25, 32–46 (1978).

  12. 12

    Cronin, M. J., Roberts, J. M. & Weiner, R. I. Endocrinology 103, 302–309 (1978).

  13. 13

    Caron, M. G. et al. J. biol. Chem. 253, 2244–2253 (1978).

  14. 14

    MacLeod, R. M. & Robyn, C. J. Endocr. 72, 273–277 (1977).

  15. 15

    Crosignani, P. G. et al. J. clin. Endocr. Metab. 45, 841–844 (1977).

  16. 16

    Clement-Cormier, Y. C., Heindel, J. J. & Robison, G. A. Life Sci. 21, 1357–1364 (1977).

  17. 17

    Rudolph, S. A., Beam, K. G. & Greengard, P. in Membrane Transport Processes Vol. 1 (ed. Hoffman, J. F.) 107–123 (Raven, New York, 1978).

  18. 18

    Jard, S., Roy, C., Barth, T., Rajerison, R. & Bockaert, J. in Advances in Cylic Nucleotide Research Vol. 5 (eds Drummond, G. J., Greengard, P. & Robison, G. A.) 31–52 (Raven, New York, 1975).

  19. 19

    Brown, E. M., Gardner, D. G., Windeck, R. A. & Aurbach, G. D. Endocrinology 103, 2323–2333 (1978).

  20. 20

    Cheung, C. J. & Weiner, R. I. Endocrinology 102, 1614–1620 (1978).

  21. 21

    Carlsson, A. in Pre- and Postsynaptic Receptors (eds Usdin, E. and Bunney, W. E.) 49–65 (Marcel Dekker, New York, 1975).

  22. 22

    Di Chiara, G., Vargiu, L., Porceddu, M. L. & Gessa, G. L. Adv. Biochem. Psychopharmac. 16, 443–447 (1977).

  23. 23

    Di Chiara, G., Corsini, G. U., Mereu, G. P., Tissari, A. & Gessa, G. L. Adv. Biochem. Psychopharmac. 19, 275–292 (1978).

  24. 24

    Ebstein, B., Roberge, C., Tabachnik, J. & Goldstein, M. J. Pharm. Pharmac. 26, 975–977 (1974).

  25. 25

    Harris, G. E., Morgenroth, V. H., Roth, R. H. & Baldessarini, R. J. Nature 252, 156–158 (1974).

  26. 26

    Roth, P. H., Walters, J. R., Murrin, L. C. & Morgenroth, V. H. III in Pre- and Postsynaptic Receptors (eds Usdin, E. & Bunney, W. E.) 5–48 (Marcel Dekker, New York, 1974).

  27. 27

    Salomon, Y., Londos, C. & Rodbell, M. Analyt. Biochem. 58, 541–548 (1974).

  28. 28

    Sinha, Y. N., Selby, F. W., Lewis, V. F. & Vanderlaan, W. P. J. clin. Endocr. Metab. 36, 509–516 (1973).

  29. 29

    Lowry, O. H., Rosenbrough, N. J., Farr, A. L. & Randall, R. J. J. biol. Chem. 193, 265–275 (1951).

  30. 30

    Mulder, G. H. & Smelik, P. G. Endocrinology 100, 1143–1152 (1977).

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

DE CAMILLI, P., MACCONI, D. & SPADA, A. Dopamine inhibits adenylate cyclase in human prolactin-secreting pituitary adenomas. Nature 278, 252–254 (1979). https://doi.org/10.1038/278252a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.