Activation of complement by cytoskeletal intermediate filaments

Abstract

INTEREST in cytoplasmic intermediate (10 nm) filaments has grown since the successful immunohistochemical differentiation of these filaments from microtubules and microfilaments1,2. Despite the widespread occurrence of intermediate filaments in various cell types, little is known about their function1,3,4. A cytoskeletal nucleus-anchoring role has been suggested based on their intracellular distribution, their connections to the Plasma membrane and nucleus, and their marked insolubility in detergents and salt solutions5,6. The presence of autoantibodies against intermediate filaments in the sera of patients7,8 suggests that these filaments can be involved in pathogenetic processes. We report here that in studies on possible pathogenetic mechanisms involving intermediate filaments, antibody-independent binding of complement components to intermediate filaments has been observed. Initial observations were made using cultured human embryonal fibroblasts, as cytoplasmic filaments present in these cells have been characterised by using both immunological markers and drugs capable of selectively altering the morphology of microfilaments, intermediate filaments and microtubules1,7. Subsequent studies showed complement binding to similar cytoplasmic structures of other cultured cells and also cells in tissue sections.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Gilbert, D. Nature, 272, 577–578 (1978).

  2. 2

    Peeters, H. (ed.) XXVIth A. Colloq. Protides of the Biological Fluids, Vol. 26 (1978).

  3. 3

    Goldman, R., Pollard, I. & Rosenbaum, J. (eds) Cell Motility (Cold Spring Harbor Laboratory, New York, 1976).

  4. 4

    Hynes, R. O. & Destree, A. T. Cell 13, 151–163 (1978).

  5. 5

    Lehto, V.-P., Virtanen, I. & Kurki, P. Nature 272, 175 (1978).

  6. 6

    Small, J. V. & Sobieszek, A. J. Cell Sci. 23, 243–268 (1977).

  7. 7

    Kurki, P., Linder, E., Virtanen, I. & Stenman, S. Nature 268, 240–241 (1977).

  8. 8

    Kurki, P., Virtanen, I., Stenman, S. & Linder, E. Clin. Immun. Immunopth. 11, 379–387 (1978).

  9. 9

    Laurila, P., Virtanen, I., Wartiovaara, J. & Stenman, S. J. Histochem. Cytochem. 26, 251–257 (1978).

  10. 10

    Yonemasu, K. & Stroud, R. S. Immunochemistry 9, 545–554 (1972).

  11. 11

    Müller-Eberhard, H. J. A. Rev. Biochem. 697–724 (1975).

  12. 12

    Lozzio, C. B. & Lozzio, B. B. Blood 45, 321–329 (1975).

  13. 13

    Cooper, N. R. & Morrison, D. C. J. Immun. 120, 1862–1868 (1978).

  14. 14

    Cooper, N. R., Jensen, F. J., Welsh, R. M. & Oldstone, M. B. A. J. exp. Med. 144, 970–984 (1976).

  15. 15

    Musoke, A. J. & Barberr, A. F. Nature 270, 438–440 (1977).

  16. 16

    Pinckard, R. N. et al. J. Immun. 110, 1376–1382 (1973).

  17. 17

    Hugh-Jones, N. C., Gardner, B. & Rowlands, J. Nature 270, 613–614 (1977).

  18. 18

    Agnello, V., Carr, R. I., Koffler, D. & Kunkel, H. G. Fedn Proc. 28, 696 (1969).

  19. 19

    Virtanen, I., Lehto, V.-P., Kurki, P., Miettinen, A. & Linder, E. Virchows Arch. (in the press).

  20. 20

    David, R. & Buchmer, A. Cancer 41, 1836–1844 (1978).

  21. 21

    Linder, E., Lehto, V.-P. & Virtanen, I. Acta path. microbiol. scand. Ser A (in the press).

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

LINDER, E., LEHTO, V. & STENMAN, S. Activation of complement by cytoskeletal intermediate filaments. Nature 278, 176–178 (1979). https://doi.org/10.1038/278176a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.