Transcription and processing of cloned yeast tyrosine tRNA genes microinjected into frog oocytes

Abstract

Xenopus oocytes transcribe cloned yeast tyrosine tRNA genes and process the RNA. The processing includes base modifications, addition of the CCA end, and splicing of the intervening sequence. The primary transcript has a 5′ leader extension which varies considerably in sequence and in length between different tRNA Tyr loci, but is nevertheless present in all of them.

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De Robertis, E., Olson, M. Transcription and processing of cloned yeast tyrosine tRNA genes microinjected into frog oocytes. Nature 278, 137–143 (1979). https://doi.org/10.1038/278137a0

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