Abstract
Xenopus oocytes transcribe cloned yeast tyrosine tRNA genes and process the RNA. The processing includes base modifications, addition of the CCA end, and splicing of the intervening sequence. The primary transcript has a 5′ leader extension which varies considerably in sequence and in length between different tRNA Tyr loci, but is nevertheless present in all of them.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Olson, M. V. et al. Nature 267, 639–641 (1977).
Hawthorne, D. L. & Leupold, U. Curr. Topics Microbiol. Immun. 64, 1–47 (1974).
Hawthorne, D. L. Biochimie 58, 179–182 (1976).
Olson, M. J., Hall, B. D., Cameron, J. R. & Davis, R. W., J. molec. Biol. (in the press).
Goodman, H. M., Olson, M. V. & Hall, B. D. Proc. natn. Acad. Sci. U.S.A. 74, 5453–5457 (1977).
Knapp, G., Beckmann, J. S., Johnson, P. F., Fuhrman, S. A. & Abelson, J. Cell 14, 221–236( 1978).
O'Farrell, P. Z., Cordell, B., Valenzuela, P., Rutter, W. J. & Goodman, H. M., Nature 274, 438–445 (1978).
Hopper, A. K., Banks, F. & Evangelidis, V. Cell 14, 211–219 (1978).
Mertz, J. E. & Gurdon, J. B. Proc. natn. Acad. Sci. U.S.A. 74, 1502–1506 (1977).
De Robertis, E. M. & Mertz, J. E. Cell 12, 175–182 (1977).
Brown, D. D. & Gurdon, J. B. Proc. natn. Acad. Sci. U.S.A. 74, 2064–2068 (1977).
Gurdon, J. B. & Brown, D. D. Devl Biol. 67, 346–356 (1978).
Kressman, A., Clarkson, S. G., Telford, J. L. & Birnstiel, M. L. Cold Spring Harb. Symp. quant. Biol. 42, 1077–1082 (1977).
Kressman, A., Clarkson, S. G., Pirrota, U. & Birnstiel, M. L. Proc. natn. Acad. Sci. U.S.A. 75, 1176–1180 (1978).
Cortese, R., Melton, D. A., Tranquilla, T. & Smith, J. D. Nucleic Acids Res. 5, 4593–4611 (1978).
Birkenmeier, E. H., Brown, D. D. & Jordan, E. Cell 15, 1077–1086 (1978).
Schmidt, O., Mao, J., Silverman, S., Hovermann, B. & Söll, D. Proc. natn. Acad. Sci. U.S.A. 75, 4819–4823 (1978).
Bolivar, F. et al. Gene 2, 95–133 (1977).
Gurdon, J. B., Melton, D. A. & De Robertis, E. M. Ciba Fdn Symp. (in the press)
Brownlee, G. G. & Sanger, F. Eur. J. Biochem. 11, 395 (1969).
Barrell, B. G. in Procedures in Nucleic Acid Research, Vol. 2 (eds Cantoni, G. L. & Davies, D. R.) pp. 751–779 (Harper and Row, New York, 1971).
Madison, J. T. & Kung, H. J. biol. Chem. 242, 1324 (1967).
Barrell, B. G. & Clark, B. F. C. Handbook of Nucleic Acid Sequences (Joynson-Bruvvers, Oxford, 1974).
Solari, A., Gatica, M. & Allende, J. E. Nucleic Acids Res. 4, 1873–1880 (1977).
Joshi, S., Haenni, A. L., Hubert, E., Huez, G. & Marbaix, G. Nature 275, 339–341 (1978).
Simoncsits, A., Brownlee, G. G., Brown, R. S., Rubin, J. R. & Guilley, H. Nature 269, 833–836 (1977).
Smith, J. D. Prog. Nucleic Acid Res. 16, 25–73 (1976).
Schultz, L. D. & Hall, B. D. Proc. natn. Acad. Sci. U.S.A. 73, 1029–1033 (1976).
Schultz, L. D. Biochemistry 17, 750–758 (1978).
Valenzuela, P., Hager, G. L., Weinberg, F. & Rutter, W. J. Proc. natn. Acad. Sci. U.S.A. 73, 1024–1028 (1976).
Sentenac, A. et al. in RNA Polymerase (eds Losick, R. & Chamberlin, M.) 763–778 (1976).
Korn, L. J. & Brown, D. D. Cell 15, 1145–1156 (1978).
Rothstein, R. J. Genetics 85, 55–64 (1977).
Di Caprio, L. & Hastings, P. J. Genetics 84, 697–721 (1976).
Capecchi, M. R., Von der Haar, R. A., Capecchi, N. E. & Sveda, M. M. Cell 12, 371–381 (1977).
Gurdon, J. B. J. Embryol. exp. Morph. 36, 523–540 (1976).
De Robertis, E. M., Partington, G. A., Longthorne, R. F. & Gurdon, J. B. J. Embryol. exp. Morph. 40, 199–214 (1977).
Sanger, F. & Coulson, A. R. FEBS Lttt. 87, 107–110 (1978).
Laskey, R. A. & Mills, A. D. FEBS Lett. 82, 314–316 (1977).
Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
De Robertis, E., Olson, M. Transcription and processing of cloned yeast tyrosine tRNA genes microinjected into frog oocytes. Nature 278, 137–143 (1979). https://doi.org/10.1038/278137a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/278137a0
This article is cited by
-
Precursors of tRNAs are stabilized by methylguanosine cap structures
Nature Chemical Biology (2016)
-
First identification of an amber nonsense mutation in Schizosaccharomyces pombe: major differences in the efficiency of homologous versus heterologous yeast suppressor tRNA genes
Molecular and General Genetics MGG (1985)
-
Intracellular transport of microinjected 5S and small nuclear RNAs
Nature (1982)
-
Impaired nuclear transport of a human variant tRNAiMet
Nature (1982)
-
tRNA precursor transcribed from a mutant human gene inserted into a SV40 vector is processed incorrectly
Nature (1982)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.