Calcium does not inhibit active sliding of microtubules from mussel gill cilia

Abstract

CALCIUM ions are probably a general regulator of the assembly state and function of most cytoplasmic microtubules, the organelles upon which processes such as cell division, transport and secretion, as well as ciliary motility, may finally depend. Ciliary activity is the best model we have of microtubule function and the cilium is the only microtubule-based system allowing extensive systematic dissection at present. We are therefore interested in the mechanism by which increasing cytoplasmic Ca2+ causes changes in ciliary beat parameters, particularly an arrest response in the mussel (Elliptio or Mytilus) gill1,2. It is well known that the beat in cilia is the result of a sliding interaction between doublet microtubules in the axoneme3,4, as can be demonstrated directly when axonemes are treated briefly with trypsin5. On addition of ATP the trypsin-treated axonemes no longer reactivate to beat normally; instead, they telescope apart. We demonstrate here that, although all sliding of the ciliary microtubules stops during ciliary arrest in vivo, the sliding interaction seen after trypsin treatment is not directly inhibited by Ca2+.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Satir, P. Science 190, 586–588 (1975).

  2. 2

    Takahashi, K. J. Fac. sci. Univ. Tokyo IV, 12, 219–228 (1971).

  3. 3

    Satir, P. J. Cell Biol. 39, 77–94 (1968).

  4. 4

    Sale, W. S. & Satir, P. Proc. natn. Acad. sci. U.S.A. 74, 2045–2049 (1977).

  5. 5

    Summers, K. E. & Gibbons, I. R. Proc. natn. Acad. sci. U.S.A. 68, 3092–3096 (1971).

  6. 6

    Walter, M. F. & Satir, P. J. Cell Biol. 79, 110–120 (1978).

  7. 7

    Tsuchiya, T. Experentia 32, 1439–1440 (1976).

  8. 8

    Schaffner, W. & Weissmann, C. Analyt. Biochem. 56, 502–514 (1973).

  9. 9

    Gibbons, B. H. & Gibbons, I. R. J. Cell Biol. 63, 970–985 (1974).

  10. 10

    Sale, W. S. thesis, Univ. California (1977).

  11. 11

    Naitoh, Y. & Kaneko, H. Science 17, 523–524 (1972).

  12. 12

    Summers, K. E. & Gibbons, I. R. J. Cell Biol. 58, 618–629 (1973).

  13. 13

    Warner, F. D. & Satir, P. J. Cell Biol. 63, 35–63 (1974).

  14. 14

    Witman, G., Fay, R. & Plummer, J. in Cell Motility (eds Goldman, R. et al.) 969–986 (Cold Spring Harbor Lab., New York, 1976).

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

WALTER, M., SATIR, P. Calcium does not inhibit active sliding of microtubules from mussel gill cilia. Nature 278, 69–70 (1979). https://doi.org/10.1038/278069a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.