Letter | Published:

Eukaryotic DNA fragments which act as promoters for a plasmid gene

Naturevolume 277pages324325 (1979) | Download Citation



TRANSCRIPTION is the process that occurs in all organisms whereby the genetic information coded by the nucleotide sequences of DNA is transferred into RNA. This process is initiated by attachment of RNA polymerase to a region of the DNA called the promoter1. Considerable information has accumulated recently concerning the structure and function of prokaryotic promoters, but little insight has been gained into the characteristics of eukaryotic promoters. We report here on experiments that use an E. coli plasmid with an attenuated promoter as a probe for possible eukaryotic promoters.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Gilbert, W. in RNA Polymerase (eds. Losick, R. & Chamberlain, M.), 193–206. (Cold Spring Harbor Laboratory, New York, 1976).

  2. 2

    Bolivar, F., Rodriguez, R., Betlach, M. & Boyer, H. Gene 2, 75–93 (1977).

  3. 3

    Rodriguez, R. L. et al. in Molecular Cloning of Recombinant DNA (eds Scott, W. A. & Werner, R.) 13, 73–84 (Academic, New York, 1977).

  4. 4

    Heberlein, G. T., DeLey, J. & Tijtgat, R. J. Bact. 94, 116–124 (1967).

  5. 5

    Valenzuela, P., Bell, G. D., Masiarz, F. R., DeGennaro, L. J. & Rutter, W. J. Nature 267, 641–643 (1977).

  6. 6

    Vapnek, D., Hautala, J. A., Jacobson, J. W., Giles, N. H. & Kushner, S. R. Proc. natn. Acad. Sci. U.S.A. 74, 3508–3512 (1977).

  7. 7

    Struhl, K., Cameron, J. R., & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. 73, 1471–1475 (1976).

Download references

Author information


  1. Department of Genetics, Briggs Hall, University of California, Davis, Davis, California, 95616

    • , ROBERT W. WEST


  1. Search for RACHAEL L. NEVE in:

  2. Search for ROBERT W. WEST in:

  3. Search for RAYMOND L. RODRIGUEZ in:

About this article

Publication history



Issue Date



Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.