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Glucocorticoids regulate the concentration of 1,25-dihydroxycholecalciferol receptors in bone

Naturevolume 277pages314315 (1979) | Download Citation

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Abstract

THE results of a study of high-affinity glucocorticoid and 1,25-dihydroxycholecalciferol (1,25(OH)2D3) binding in cytosol from fetal rat calvaria cultured for up to 48 h at 37°C are presented. The binding of glucocorticoids was found to be stable in short-term culture, whereas that for 1,25(OH)2D3 declined rapidly, was almost undetectable at 24 h and had disappeared by 48 h. Furthermore, low concentrations of cortisol (10−8 to 10−6 M) added to the culture medium had a dramatic effect on the persistence of high affinity 1,25(OH)2D3 binding. In the presence of cortisol there was dose-dependent preservation of most of the binding of 1,25(OH)2D3 in the cytosol. 1,25(OH)2D3 is a potent agent in inducing bone resorption in vitro and these data suggest that glucocorticoids may exert part of their effects on calcium metabolism and bone in vivo by directly regulating the turnover of cytosol 1,25(OH)2D3 receptors in one or more types of bone cell.

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References

  1. 1

    Manolagas, S. C. & Anderson, D. C. J. Endocr. 76, 379–380 (1978).

  2. 2

    Manolagas, S. C., Taylor, C. M. & Anderson, D. C. J. Endocr. 80, 35–39 (1979).

  3. 3

    Feldman, D., Dziak, R., Koehler, R. & Stern, P. Endocrinology 96, 29–36 (1975).

  4. 4

    Kream, B. E., José, M., Yamada, S. & DeLuca, H. F. Science 197, 1086–1088 (1977).

  5. 5

    Chen, T. L. & Feldman, D. Endocrinology 102, 236–244 (1978).

  6. 6

    Harrison, H. E. & Harrison, H. C. Am. J. Physiol. 199, 265–271 (1960).

  7. 7

    Jowsey, J., Kelly, P. J., Riggs, B. L., Bianco, A. J. & Scholz, D. A. J. Bone Joint Surg. 47 B, 785–806 (1965).

  8. 8

    Avioli, L. V., Birge, S. J. & Lee, S. W. J. clin. endocr. Metab. 28, 1341–1346 (1968).

  9. 9

    Kimberg, D. V., Baerg, R. D., Gershon, E. & Graudusius, R. T. J. clin. Invest. 50, 1309–1321 (1971).

  10. 10

    Raisz, L. G., Trummel, C. L., Wener, J. A. & Simmons, H. Endocrinology 90, 961–967 (1972).

  11. 11

    Carré, M., Ayigbede, O., Miravet, L. & Rasmussen, H. Proc. natn. Acad. Sci., U.S.A. 71, 2996–3000 (1974).

  12. 12

    Klein, R. G., Arnaud, S. B., Gallagher, J. C., DeLuca, H. F. & Riggs, B. L. J. clin. Invest. 60, 253–259 (1977).

  13. 13

    Spanos, E. & MacIntyre, I. in Vitamin D Biochemical and Clinical Aspects Related to Calcium Metabolism (ed. Norman, A. W.) 369–371 (de Gruter, Berlin, 1977).

  14. 14

    Lukert, B. D., Stanbury, S. W. & Mawer, E. B. Endocrinology 93, 718–722 (1973).

  15. 15

    Wong, G. L., Luben, R. A. & Cohn, D. V. Science 197, 663–665 (1977).

  16. 16

    Reynolds, J. J. Biochem. Soc. Spec. Publ. 3, 91–102 (1974).

  17. 17

    DeLuca, H. F. & Schnoes, H. K. A. Rev. Biochem. 45, 631 (1976).

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Affiliations

  1. Department of Medicine, Hope Hospital, University of Manchester School of Medicine, Eccles Old Road, Salford, UK

    • S. C. MANOLAGAS
    •  & D. C. ANDERSON
  2. Department of Medicine, Manchester Royal Infirmary, University of Manchester School of Medicine, Manchester, UK

    • G. A. LUMB

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https://doi.org/10.1038/277314a0

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