Letter | Published:

Dexamethasone-induced adhesion in hepatoma cells: the role of plasminogen activator

Naturevolume 277pages312313 (1979) | Download Citation

Subjects

Abstract

TRANSFORMED CELLS are frequently less adhesive than normal cells. This decrease in adhesion has been associated with the loss of specific cell surface proteins, changes in morphology, alterations in the underlying cytoskeleton and the increased activity of the serine protease, plasminogen activator1–3. Hepatoma tissue culture (HTC) cells exhibit increased levels of adhesion to glass4,5 and decreased plasminogen activator activity6,7 when incubated in the presence of the synthetic glucocorticoid, dexamethasone. Plasminogen activator converts plasminogen in serum to plasmin. It has been shown that plasmin causes the reversible dissociation of intracellular actin-containing cables in anchorage-dependent rat embryo cells8 and may also be partially responsible for decreased adhesion of Rous sarcoma virus-transformed chicken embryo fibroblasts3. Cell–substrate adhesion presumably requires specific cell surface glycoproteins and intact cytoskeletal elements9,10. It is possible that plasmin acts either to modify ‘adhesive’ proteins on the cell surface or to disrupt linkages between these surface components and the underlying cytoskeleton. We have used variant HTC cells selectively resistant to the dexamethasone inhibition of plasminogen activator6 to test the hypothesis that hormonal induction of cell adhesion is secondary to the inhibition of plasminogen activator which in turn allows the accumulation of the glycoproteins necessary for adhesion. We have modified cell adhesion assay techniques5,11 to provide a sensitive measure of cell–substrate adhesion for both wild-type and variant HTC cells in the presence and absence of serum and plasminogen. We report here that plasminogen activator does not have an important role in the hormonal regulation of HTC cell adhesiveness.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Shields, R. & Pollock, K. Cell 3, 31–38 (1974).

  2. 2

    Unkeless, J. C. et al. J. exp. Med. 137, 85–111 (1973).

  3. 3

    Weber, M. J. Cell 5, 253–261 (1975).

  4. 4

    Ballard, P. L. & Tomkins, G. M. Nature 224, 344–345 (1969).

  5. 5

    Ballard, P. L. & Tomkins, G. M. J. Cell Biol. 47, 222–234 (1970).

  6. 6

    Carlson, S. A. & Gelehrter, T. D. J. supramolec. Struct. 6, 325–331 (1977).

  7. 7

    Wigler, M., Ford, J. P. & Weinstein, I. B. in Proteases and Biological Control, 849–856 (Reich, E, Rifkin, D. B. & Shaw, E. eds) (Cold Spring Harbor Press, New York, 1975).

  8. 8

    Pollack, R. & Rifkin, D. Cell 6, 495–506 (1975).

  9. 9

    Rees, D. A., Lloyd, C. W. & Thom, D. Nature 267, 124–128 (1977).

  10. 10

    Willingham, M. C., Yamada, K. M., Poussegur, J. & Pastan, I. Cell 10, 375–380 (1977).

  11. 11

    Walther, B. T., Ohman, P. & Roseman, S. Proc. Natn. Acad. Sci. U.S.A. 70, 1569–1573 (1973).

  12. 12

    Spencer, C. J. & Gelehrter, T. D. J. biol. Chem. 249, 577–583 (1974).

  13. 13

    Culp. L. A. & Buniel, J. F. J. cell. Physiol. 88, 89–106 (1976).

  14. 14

    Grinnell, F. Archs Biochem. Biophys. 169, 474–482 (1975).

  15. 15

    Ivarie, R. D. & O'Farrell, P. H. Cell 13, 41–55 (1978).

  16. 16

    Deutsch, D. G. & Mertz, E. T. Science 170, 1095–1096 (1970).

  17. 17

    Strickland, S. & Beers, W. H. J. biol. Chem. 251, 5694–5702 (1976).

Download references

Author information

Author notes

    • BARBARA L. FREDIN

    Present address: Department of Biology, Indiana University, Bloomington, Indiana, 47401

    • SARAH CARLSON SEIFERT

    Present address: Department of Internal Medicine, University of Iowa, Iowa City, Iowa, 52252

Affiliations

  1. Departments of Human Genetics and Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan, 48109

    • BARBARA L. FREDIN
    • , SARAH CARLSON SEIFERT
    •  & THOMAS D. GELEHRTER

Authors

  1. Search for BARBARA L. FREDIN in:

  2. Search for SARAH CARLSON SEIFERT in:

  3. Search for THOMAS D. GELEHRTER in:

About this article

Publication history

Received

Accepted

Issue Date

DOI

https://doi.org/10.1038/277312a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.