Letter | Published:

Formation of a new fluorophore on irradiation of carboxymethylated D-glyceraldehyde-3-phosphate dehydrogenase

Naturevolume 277pages245246 (1979) | Download Citation



BECAUSE of its physiological importance, allosteric properties and ready availability, D-glyceraldehyde-3-phosphate dehydrogenase (G3PDH) is one of the most thoroughly studied of the dehydrogenases. We report here a study of the ligand binding properties of G3PDH. We observed that when active site carboxymethylated G3PDH was irradiated with near ultraviolet light in the presence of NAD+, a new fluorophore was formed.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Harris, J. I., Meriwether, B. P. & Park, J. H. Nature 198, 154–157 (1963).

  2. 2

    Spies, J. R. Analyt. Chem. 39, 1412–1416 (1967).

  3. 3

    Moras, D. et al. J. biol. Chem. 250, 9139–9162 (1975).

  4. 4

    Biesecker, G., Harris, J. I., Thierry, J. G. & Walker, J. E. Nature 266, 328–333 (1977).

  5. 5

    Bolotina, I. A., Markovich, D. S., Volkenstein, M. V. & Zavodsky, P. Biochim. biophys. Acta 132, 260–270 (1967).

  6. 6

    Kaplan, N. O. The Enzymes 3, 2nd edn 130–146 (1960).

  7. 7

    Cilento, G. & Tedeschi, P. J. biol. Chem. 236, 907–910 (1961).

  8. 8

    Shifrin, S. Biochim. biophys. Acta 81, 205–213 (1964).

  9. 9

    Ho, Y. S. et al. Scientia Sinica (submitted).

Download references

Author information


  1. Institute of Biophysics, Academia Sinica, Peking, China

    • YEN-SEN HO
    •  & CHEN-LU TSOU


  1. Search for YEN-SEN HO in:

  2. Search for CHEN-LU TSOU in:

About this article

Publication history



Issue Date




By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.