Letter | Published:

Acetylcholine receptors in organ-cultured human muscle fibres

Abstract

ORGAN culture of nerve and muscle facilitates investigations which cannot easily be carried out in situ1,2. We have developed a method for maintaining human muscle in organ culture which should allow us to investigate normal as well as various pathological properties of muscle fibres. We report here the use of these preparations to compare junctional and extrajunctional channels, induced by acetylcholine (ACh), in human muscle. In addition we have made a preliminary study of miniature endplate currents, in normal and myasthenia gravis endplates, following organ culture.

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