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Immunochemical quantification of in vitro neutrophilic granulocyte differentiation

Naturevolume 277pages225227 (1979) | Download Citation

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Abstract

IN the presence of colony stimulating activity (CSA)1–5, both normal and neoplastic cells of the neutrophilic granulocyte (neutrophil) and monocyte–macrophage cell series proliferate and differentiate in semi-solid and liquid cultures6–9. However, as conventionally performed, the clonal assay for CSA depends on cellular proliferation, and does not provide quantitative information on the role of CSA in differentiation. Lactoferrin (LF) is a specific protein marker for the neutrophil cell series10–12, and among bone marrow cells is found exclusively in the specific granules of the maturing neutrophil13,14. These granules first appear at the myelocyte stage of cytodifferentiation15. Thus, synthesis of LF is a biochemical event which may be used to quantify a specific state of neutrophil differentiation. We previously reported the purification of murine LF16, and its quantification by radioimmunoassay17, and also the development of a slide chamber method for culturing bone marrow cells in liquid medium18. We now report that in similar cultures, the net synthesis of LF is correlated in a dose-dependent manner with CSA concentration. To our knowledge, this is the first demonstration of a quantitative relationship at both the biochemical and cellular levels between CSA and a specific marker of neutrophilic granulocyte differentiation19.

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Affiliations

  1. Department of Biochemistry, Emory University School of Medicine

    • JOSEPH M. KINKADE JR
    •  & KATHRYN L. KELLAR
  2. Department of Medicine, Division of Hematology and Oncology, Emory University School of Medicine, Atlanta, Georgia, 30322

    • ELLIOTT F. WINTON

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https://doi.org/10.1038/277225a0

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