Letter | Published:

X-ray absorption edge fine structure spectroscopy of the active site haem of cytochrome c

Naturevolume 277pages150151 (1979) | Download Citation

Subjects

Abstract

THE new technique of extended X-ray absorption fine structure spectroscopy (EXAFS)1 offers promise of detecting small changes in conformation around a central metal atom at the active site of a metalloenzyme, and of relating such changes to the catalytic mechanism. An advantage of EXAFS studies over X-ray diffraction is that they can be carried out in solution at physiological pH where the protein can freely interact with its substrate. We describe here EXAFS measurements aimed at detecting conformational changes during reduction of horse heart cytochrome c.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Stern, E. A. Sci. Am. 234, 96–103 (1976).

  2. 2

    Dickerson, R. E. et al. J. biol. Chem. 264, 1511–1535 (1971).

  3. 3

    Smith, L., Davies, H. C., Reichlin, M. & Margoliash, E. J. biol. Chem. 248, 237–243 (1973).

  4. 4

    Takano, T. et al. J. biol. Chem. 252, 776–785 (1977).

  5. 5

    Takano, T., Kallai, O. B., Swanson, R. & Dickerson, R. E. J. biol. Chem. 248, 5234–5255 (1973).

  6. 6

    Andersen, N. Geophysics 39, 69–72 (1974).

  7. 7

    Ulrych, T. J., Bishop, T. N. Rev. Geophys. Space. Phys. 13, 183–200 (1975).

  8. 8

    Yuen, C., Weissbluth, M. & Labhardt, A. M. SSRP-Report No. 77/06 (1977).

  9. 9

    Ulmer, D. D., Kägi, J. H. R. Biochemistry 7, 2710–2717 (1968).

  10. 10

    Cohen, J. S., Fisher, W. R. & Schechter, A. N. J. biol. Chem. 249, 1113–1118 (1974).

  11. 11

    Harbury, H. A. et al. Proc. natn. Acad. Sci. U.S.A. 54, 1658–1664 (1965).

  12. 12

    Wilgus & Stellwagen, E. Proc. natn. Acad. Sci. U.S.A. 71, 2892–2894 (1974).

  13. 13

    Shulman, R. G., Yafet, Y., Eisenberger, P. & Blumberg, E. Proc. natn. Acad. Sci. U.S.A. 73, 1384–1388 (1976).

  14. 14

    Greenwood, C. & Palmer, G. J. biol. Chem. 240, 3660–3663 (1965).

Download references

Author information

Author notes

    • A. LABHARDT

    Present address: Department of Biophysical Chemistry, Biocentre of the University of Basel, Switzerland

Affiliations

  1. Department of Biochemistry, Stanford University Medical Center, Stanford, California, 94305

    • A. LABHARDT
  2. Department of Physics, Stanford University, Stanford, California, 94305

    • C. YUEN

Authors

  1. Search for A. LABHARDT in:

  2. Search for C. YUEN in:

About this article

Publication history

Received

Accepted

Issue Date

DOI

https://doi.org/10.1038/277150a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.