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Activation of adenylate cyclase by vanadate

Abstract

VANADATE has been identified as a potent inhibitor of (Na,K)ATPases1,2. The (Na,K)ATPase of dog kidney is inhibited 50% by 40 nM sodium orthovanadate (Na2VO4) in optimal conditions (28 mM Mg2+)2. As vanadate was originally identified as an inhibitor in muscle-derived ATP preparations2 and in equine and rabbit skeletal muscles, it has been suggested as endogenous regulator of (Na,K)ATPase2–4. We have tried to use vanadate as a tool to inhibit ATPases in adenylate cyclase studies when working at low ATP concentrations. We report here that vanadate causes marked stimulation of adenylate cyclase activity in membranes isolated from rat fat cells and produces maximal activation even in the absence of hormones.

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SCHWABE, U., PUCHSTEIN, C., HANNEMANN, H. et al. Activation of adenylate cyclase by vanadate. Nature 277, 143–145 (1979). https://doi.org/10.1038/277143a0

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