Letter | Published:

Identification of the neuritogen for experimental allergic neuritis

Naturevolume 277pages140142 (1979) | Download Citation



EXPERIMENTAL ALLERGIC NEURITIS (EAN) is a model of human acute inflammatory polyneuropathy, Guillain–Barré syndrome. It can be produced in several species of laboratory animal by immunisation with homologous or heterologous peripheral nerve emulsified in Freund's complete adjuvant1,2. After about two weeks the animals may develop signs varying from ataxia to paralysis and death. EAN has been produced using crude acid extracts of peripheral nerve3,4, but investigations into the neuritogenic properties of the component proteins of peripheral nerve myelin have yielded inconclusive results5–10. In experimental allergic encephalomyelitis (EAE), the central nervous system (CNS) counterpart of EAN, the encephalitogenic antigen has been shown to be a CNS myelin basic protein of molecular weight 18,000 (ref. 11). The three major proteins of peripheral nerve myelin, which together account for about 75% of the myelin protein, are usually referred to as P0, P1 and P2 (ref. 12). P0 is a glycoprotein of molecular weight 30,000 and P1 and P2 are both basic proteins with molecular weights of 18,000 and 12,000–14,000, respectively12–13. We describe here a reliable method for producing severe EAN in inbred Lewis rats with bovine intradural root material, and show that intact P2, the major basic protein of peripheral nerve myelin, is the responsible neuritogen.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Waksman, B. H. & Adams, R. D. J. exp. Med. 102, 213–235 (1955).

  2. 2

    Waksman, B. H. & Adams, R. D. J. Neuropath. exp. Neurol. 15, 293–332 (1956).

  3. 3

    Behan, P. O., Lamarcke, J. B., Behan, W. M., Feldman, R. G. & Kies, M. W. Trans. Am. neural. Ass. 94 219–222 (1969).

  4. 4

    Sheremata, W. A. & Behan, P. O. J. Neurol. Neurosurg. Psychiat. 36, 139–145 (1973).

  5. 5

    Brostoff, S., Burnett, P., Lampert, P. & Eylar, E. H. Nature new Biol. 235, 210–212 (1972).

  6. 6

    Uyemura, K., Tobari, C., Hirano, S. & Tsukada, T. J. Neurochem. 19, 2706–2614 (1972).

  7. 7

    Brostoff, S. W., Karkhains, Y. D., Carlo, D. J., Reuter, W. & Eylar, E. H. Brain Res. 86, 449–458 (1975).

  8. 8

    Abramsky, O., Teitelbaum, D., Webb, C. & Arnon, R. J. Neuropath. exp. Neurol. 34, 36–45 (1975).

  9. 9

    Abramsky, O., Teitelbaum, D. & Arnon, R. Eur. J. Immun. 7, 213–217 (1977).

  10. 10

    Brostoff, S. W., Levit, S. & Powers, J. M. Nature 268, 752–753 (1977).

  11. 11

    Laatsch, B. A., Kies, M. W., Gordon, S. & Alvord, E. C. J. exp. Med. 115, 777–788 (1962).

  12. 12

    Greenfield, S., Brostoff, S., Eylar, E. H. & Morell, P. J. Neurochem. 20, 1207–1216 (1973).

  13. 13

    Deibler, G. E., Driscoll, B. P. & Kies, M. W. J. Neurochem. 30, 401–412 (1978).

  14. 14

    Ishaque, A., Roomi, M. W., Khan, N. R. & Eylar, E. H. Biochim. biophys. Acta 495, 77–86 (1977).

  15. 15

    Norton, W. T. & Paduslo, S. E. J. Neurochem. 21, 759–773 (1973).

  16. 16

    Kitamura, K., Yamanaka, T. & Uyemura, K. Biochim. biophys. Acta 379, 582–591 (1975).

  17. 17

    Smith, M. E., Forno, L. S. & Hofmann, W. W. Trans. Am. Soc. Nerurochem. 9, 55 (1978).

  18. 18

    Brostoff, S. W. & Eylar, E. H. Archs. Biochem. Biophys. 153, 590–598 (1972).

Download references

Author information


  1. Department of Medicine, Guy's Hospital Medical School, London Bridge, London, SE1, UK

    •  & R. A. C. HUGHES


  1. Search for M. KADLUBOWSKI in:

  2. Search for R. A. C. HUGHES in:

About this article

Publication history



Issue Date



Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.