Letter | Published:

Rat × rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG

Naturevolume 277pages131133 (1979) | Download Citation

Subjects

Abstract

MONOCLONAL antibodies can be produced by cultures of permanent cell lines derived by fusion of suitable myelomas with spleen cells from immunised animals1. So far, mainly two mouse myelomas, X63-Ag8 and NSI/1-Ag4.1, have been used for this purpose1,2. When cells from immunised mice are used in the fusion, the mouse × mouse hybrid myelomas can be grown as tumours in appropriate mouse strains. We describe here the preparation and use of a new clone of a rat myeloma, which is suitable for the derivation of rat × rat hybrid myelomas producing specific rat antibodies. The spent medium of hybrid myeloma cultures usually contains 1–20 μg ml−1 antibody. The serum and ascites of the tumour-bearing mice, with few exceptions, yield 1–20 mg ml−1 of antibody, which is 1,000 times more concentrated and very convenient for larger preparations.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Köhler, G. & Milstein, C. Nature 256, 495–497 (1975).

  2. 2

    Köhler, G. & Milstein, C. Eur. J. Immun. 6, 511–519 (1976).

  3. 3

    Galfrè, G., Howe, S. C., Milstein, C., Butcher, G. W. & Howard, J. C. Nature 266, 550–552 (1977).

  4. 4

    Springer, T., Galfrè, G., Secher, D. S. & Milstein, C. Eur. J. Immun. 8, 539–551 (1977).

  5. 5

    Bazin, H., Deckers, C., Beckers, A. & Heremans, J. F. Int. J. Cancer 10, 568 (1972).

  6. 6

    Cotton, R. G. H. & Milstein, C. Nature 244, 42–43 (1973).

  7. 7

    Svasti, J. & Milstein, C. Biochem. J. 128, 427–444 (1972).

  8. 8

    Secher, D. S., Milstein, C. & Adetugbo, K. Immun. Rev. 36, 51–72 (1977).

  9. 9

    Milstein, C., Adetugbo, K., Cowan, N. J. & Secher, D. S. Prog. Immun. 2, 157–168 (1974).

  10. 10

    Rocca-Serra, J., Milili, M. & Fougereau, M. Eur. J. Biochem. 59, 511–523 (1975).

  11. 11

    Turner, K. J. & Cebra, J. J. Biochemistry 10, 9–17 (1971).

  12. 12

    Fruchter, R. G., Jackson, S. A., Mole, L. E. & Porter, R. R. Biochem. J. 116, 249–259 (1970).

  13. 13

    Edelman, G. M. et al. Proc. natn. Acad. Sci. U.S.A. 63, 78–85 (1969).

  14. 14

    Poljak, R. J., Amzel, L. M., Chen, B. L., Phizackerley, R. P. & Saul, F. Proc. natn. Acad. Sci. U.S.A. 71, 3440 (1974).

Download references

Author information

Affiliations

  1. MRC Laboratory of Molecular Biology, Hills Road, Cambridge, UK

    • G. GALFRÈ
    • , C. MILSTEIN
    •  & B. WRIGHT

Authors

  1. Search for G. GALFRÈ in:

  2. Search for C. MILSTEIN in:

  3. Search for B. WRIGHT in:

About this article

Publication history

Received

Accepted

Issue Date

DOI

https://doi.org/10.1038/277131a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.