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Synthesis of rabbit β-globin in cultured monkey kidney cells following infection with a SV40 β-globin recombinant genome

Naturevolume 277pages108114 (1979) | Download Citation

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Abstract

Rabbit β-globin complementary DNA (cDNA) has been inserted into SV40DNA in place of the gene coding for the virus' major capsid protein, VP1. The recombinant genome, SVGT5-RaβG, multiplies efficiently in CV1 monkey kidney cell cultures and is transcribed to yield cytoplasmic, polyadenylated hybrid mRNAs containing the β-globin coding sequence. Cells propagating SVGT5-RaβG produce substantial quantities of rabbit β-globin polypeptide.

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  1. Department of Biochemistry, Stanford University Medical Center, Stanford, California, 94305

    • Richard C. Mulligan
    • , Bruce H. Howard
    •  & Paul Berg

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https://doi.org/10.1038/277108a0

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