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Adenosine inhibits the accumulation of cyclic AMP in cultured brain cells


ADENOSINE stimulates the accumulation of cyclic AMP in some tissues and cell types, but has inhibitory or biphasic effects on the accumulation of cyclic AMP in other cells. Londos and Wolff1 have attempted to explain these diverse effects by the existence of two adenosine reactive sites on adenylate cyclase. The first is the R-site, occupancy of which usually leads to activation on adenylate cyclase and which for activity requires integrity of the ribose ring. Much evidence suggests that this site is identical to an extracellular receptor for adenosine2–6. The second type is the P-site, which mediates inhibition of adenylate cyclase and requires integrity of the purine ring for activity. Preliminary evidence suggests that this site is only accessible from the interior of the cell1,3. However, we report here that the inhibition by adenosine of the increase in the intracellular level of cyclic AMP evoked by isoprenaline in cultured glioblasts is mediated by extracellular receptors.

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CALKER, D., MÜLLER, M. & HAMPRECHT, B. Adenosine inhibits the accumulation of cyclic AMP in cultured brain cells. Nature 276, 839–841 (1978).

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