Abstract
12-O-TETRADECANOYL PHORBOL-13-ACETATE (TPA), a potent tumour-promoting agent, stimulates the proliferation of various cell types, including fibroblasts, in cell culture (see ref. 1 for review). Recent studies have demonstrated that there is a close correlation between the potency of phorbol esters for promoting skin tumours and their ability to stimulate DNA synthesis in cultured cells2. The growth-stimulating activity of TPA is completely dependent on the presence of serum in the nutrient medium. This requirement for serum has been demonstrated in cultures of mouse epidermal cells3 and fibroblastic cells4,5. The role of serum in the cellular response to TPA remains unresolved. We report here that serum can be replaced by polypeptide hormones like epidermal growth factor (EGF)6, and insulin and by the growth factor isolated from the conditioned medium of SV40 BHK cells (fibroblast-derived growth factor, FDGF)7. Low concentrations of TPA and the growth-factors stimulate DNA synthesis in quiescent cultures of mouse and human cells in completely serum-free medium.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Slaga, T. J., Sivak, A. & Boutwell, R. K. (eds) Mechanisms of tumor promotion and cocarcinogenesis (Raven, New York, 1978).
Yuspa, S. H. et al. Nature 262, 402–404 (1976).
Yuspa, S. H., Licht, U., Hennings, H., Ben, T., Patterson, E. & Slaga, T. J. in Mechanisms of tumor promotion and carcinogenesis (eds Slaga, T. J., Sivak, A. & Boutwell, R. K.) 245–256 (Raven, New York, 1978).
Sivak, A. J. cell. Physiol. 80, 167–174 (1972).
Boynton, A. L., Whitfield, J. E. & Isaacs, R. J. J. cell. Physiol. 87, 25–32 (1976).
Savage, C. R. Jr, Inagomi, T. & Cohen, S. J. biol. Chem. 247, 7612–7621 (1972).
Bourne, H. & Rozengurt, E. Proc. natn. Acad. Sci. U.S.A. 73, 4555–4559 (1976).
Pledger, W. J., Stiles, C. D., Antoniades, H. N. & Scher, C. D. Proc. natn. Acad. Sci. U.S.A. 74, 4481–4485 (1977).
Fisher, P. B., Weinstein, I. B., Eisenberg, O. & Ginsberg, H. S. Proc. natn. Acad. Sci. U.S.A. 75, 2311–2314 (1978).
Mondal, S. & Heidelberger, C. Nature 260, 710–711 (1976).
Cohen, R., Pacifici, M., Rubinstein, N., Biehl, J. & Holtzer, H. Nature 266, 538–540 (1977).
Rovera, G., O'Brien, T. G. & Diamond, L. Proc. natn. Acad. Sci. U.S.A. 74, 2894–2898 (1977).
Diamond, L., O'Brien, S., Donaldson, C. & Shimiza, Y. Int. J. Cancer 13, 721–730 (1974).
Mierzejewski, K. & Rozengurt, E. Expl Cell. Res. 106, 394–397 (1977).
Rozengurt, E. & Heppel, L. A. Proc. natn. Acad. Sci. U.S.A. 72, 4492–4495 (1975).
Mierzejewski, K. & Rozengurt, E. Biochem. Biophys. Res. Commun. 73, 271–278 (1976).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
DICKER, P., ROZENGURT, E. Stimulation of DNA synthesis by tumour promoter and pure mitogenic factors. Nature 276, 723–726 (1978). https://doi.org/10.1038/276723a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/276723a0
This article is cited by
-
TGF-β and phorbol esters inhibit mitogenesis utilizing parallel protein kinase C-dependent pathways
Kidney International (1995)
-
Media for cultivation of animal cells: an overview
Cytotechnology (1988)
-
The Na−K−2Cl cotransport system
The Journal of Membrane Biology (1986)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.