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Transfer of the drug-resistance transposon Tn5 to Rhizobium


TRANSPOSONS are discrete sequences of DNA that are incapable of self-replication and can insert into DNA replicons, such as chromosomes or plasmids, in the absence of the recA gene function1. Insertion can be random and where it occurs into the continuity of a gene leads to non-leaky polar mutations1. Some transposons carry drug resistance genes and thus insertions can be mapped as drug-resistance markers1. We report here that Tn5, a transposon coding for kanamycin resistance2, can insert into the chromosome of Rhizobium leguminosarum, the bacterium responsible for nodulation and nitrogen fixation in Pisum, Vicia, Lathyrus and Lens.

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  1. Kleckner, N., Roth, J. & Botstein, D. J. molec. Biol. 116, 125–159 (1977).

    Article  CAS  Google Scholar 

  2. Berg, D. E., Davies, J., Allet, B. & Rochaix, J.-D. Proc. natn. Acad. Sci. U.S.A. 72, 3628–3632 (1975).

    Article  CAS  ADS  Google Scholar 

  3. Datta, N., Hedges, R. W., Shaw, E. J., Sykes, R. B. & Richmond, M. H. J. Bact. 108, 1244–1249 (1971).

    CAS  PubMed  Google Scholar 

  4. Olsen, R. H. & Shipley, P. J. Bact. 113, 772–780 (1973).

    CAS  PubMed  Google Scholar 

  5. Beringer, J. E., Hoggan, S. A. & Johnston, A. W. B. J. gen. Microbiol. 104, 201–207 (1978).

    Article  Google Scholar 

  6. Meade, H. M. & Signer, E. R. Proc. natn. Acad. Sci. U.S.A. 74, 2076–2078 (1977).

    Article  CAS  ADS  Google Scholar 

  7. Kondorosi, A., Kiss, G. B., Forrai, T., Vincze, E. & Banfalvi, Z. Nature 268, 525–527 (1977).

    Article  ADS  Google Scholar 

  8. Towner, K. J. & Vivian, A. J. gen. Microbiol. 101, 167–171 (1977).

    Article  CAS  Google Scholar 

  9. Boucher, C., Bergeron, B., Barate de Bertalmio, M. & Dénarié, J. J. gen. Microbiol. 98, 253–263 (1977).

    Article  CAS  Google Scholar 

  10. Van Vliet, F., Silva, B., van Montagu, M. & Schell, J. Plasmid (in the press).

  11. Hernalsteens, J. P., De Greve, M., van Montagu, M. & Schell, J. Plasmid 1, 218–225 (1978).

    Article  CAS  Google Scholar 

  12. Hirsch, P. R. thesis, Univ. East Anglia (1978).

  13. Howe, M. M. & Bade, E. G. Science 190, 624–632 (1975).

    Article  CAS  ADS  Google Scholar 

  14. Johnston, A. W. B., Beynon, J. L., Buchanan-Wollaston, V. A., Setchell, S. M., Hirsch, P. R. & Beringer, J. E. Nature, accompanying paper (1978).

  15. Johnston, A. W. B. & Beringer, J. E. J. gen. Microbiol. 87, 343–350 (1975).

    Article  CAS  Google Scholar 

  16. Johnston, A. W. B., Setchell, S. M. & Beringer, J. E. J. gen. Microbiol. 104, 209–218 (1978).

    Article  Google Scholar 

  17. Ely, B. & Johnson, R. C. Genetics 87, 391–399 (1977).

    CAS  PubMed  PubMed Central  Google Scholar 

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BERINGER, J., BEYNON, J., BUCHANAN-WOLLASTON, A. et al. Transfer of the drug-resistance transposon Tn5 to Rhizobium. Nature 276, 633–634 (1978).

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